A General Method for Measuring Persister Levels in Escherichia coli Cultures

Methods in Molecular Biology
Niilo KaldaluTanel Tenson

Abstract

Genetically homogeneous bacterial cultures contain persisters, cells that are not killed by bactericidal antibiotics. These cells are suggested to be involved in the establishment of chronic infections. Persister levels depend on growth conditions. Here, we discuss the parameters that have to be considered when measuring persister levels and provide a sample protocol to do it.

Citations

Nov 13, 2020·Microbiology and Molecular Biology Reviews : MMBR·Niilo KaldaluTanel Tenson

Related Concepts

Bactericide, NOS
Escherichia coli
Chronic Infectious Disease
Bacterial Culture (Procedure)

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