A general route to fingerprint analyses of peptide-antibody interactions using a clustered amino acid peptide library: comparison with a phage display library

Molecular Immunology
A KramerJ Schneider-Mergener

Abstract

We provide a general route to fingerprint analyses of peptide-antibody interactions using a novel chemically synthesized peptide library. A combinatorial clustered amino acid peptide library XO1O2O3O4X (O = one of six amino acid clusters [APG], [DE], [HKR], [NQST], [FYW] and [ILVM]; X = randomized position) bound to a continuous cellulose membrane support was designed to overcome the problem of combinatorial explosion in the synthesis of peptide libraries. This library served as the starting point for the identification and detailed characterization of a TGF alpha peptide epitope recognized by the antibody Tab2. By analysing 1728 hexapeptide mixtures and 1600 single hexapeptides we identified a large number of structurally different high affinity Tab2 binding molecules. Our data provide a detailed picture of the structural basis of this antibody-peptide interaction. In addition to the detection of key amino acids involved in Tab2 binding we observed a high variability of Tab2 binding sequences supporting an induced fit mechanism in antibody-peptide recognition. In contrast, a phage display hexapeptide library led to the detection of only one dominant Tab2 binding peptide. The data obtained also demonstrate the influence of phag...Continue Reading

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Citations

Aug 27, 1998·Molecular Biotechnology·F al-ObeidiT K Sawyer
Jun 1, 1996·Immunotechnology : an International Journal of Immunological Engineering·A KolaJ Köhl
Jul 24, 2002·Journal of Immunological Methods·Ulrich ReinekeJens Schneider-Mergener
Apr 1, 1997·Current Opinion in Immunology·M S HaydenJ A Ledbetter
May 14, 1999·Current Opinion in Immunology·C PinillaR A Houghten
Oct 26, 1999·The Journal of Peptide Research : Official Journal of the American Peptide Society·A KramerJ Schneider-Mergener
Dec 7, 2007·Biotechnology & Genetic Engineering Reviews·Kai HilperRobert E W Hancock
Oct 3, 1999·Journal of Medicinal Chemistry·R A HoughtenJ M Ostresh
Aug 1, 1997·Analytical Chemistry·Y V LyubarskayaB L Karger

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