A general strategy for identification of S-layer genes in the Bacillus cereus group: molecular characterization of such a gene in Bacillus thuringiensis subsp. galleriae NRRL 4045

Microbiology
S MesnageA Fouet

Abstract

Despite its possible role in virulence, there has been little molecular characterization of members of the S-layer protein family of the Bacillus cereus group. It is hypothesized that the components of the S-layers are likely to display similar anchoring structures in Bacillus thuringiensis and Bacillus anthracis. Based on inferred sequence similarities, a DNA fragment encoding the cell-wall-anchoring domain of an S-layer component of BAC: thuringiensis subsp. galleriae NRRL 4045 was isolated. The complete gene was identified and sequenced. An ORF of 2463 nt was identified, which was predicted to encode a protein of 821 aa, SlpA. The mature SlpA protein (792 residues) carries three S-layer homology (SLH) motifs towards its amino terminus, each about 50 aa long. These motifs were sufficient to bind Bac. thuringiensis and Bac. anthracis cell walls in vitro by interacting with peptidoglycan-associated polymers, confirming a common wall-anchoring mechanism. The slpA null-allele mutant was constructed and shown to possess no other abundant surface protein. It is proposed that the method described in this paper could be applied to the identification and deletion of any Bac. cereus S-layer gene and is of great value for the molecular ...Continue Reading

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Citations

Jul 18, 2009·Molecular Aspects of Medicine·Agnès Fouet
Jul 12, 2005·Biotechnology Advances·K PollmannS Selenska-Pobell
May 29, 2012·Applied and Environmental Microbiology·César de la Fuente-NúñezRobert E W Hancock
May 7, 2003·Applied and Environmental Microbiology·Lyndsay RadnedgeGary L Andersen
Sep 4, 2019·Investigative Ophthalmology & Visual Science·Md Huzzatul MursalinMichelle C Callegan
Jan 5, 2006·Applied and Environmental Microbiology·Guadalupe PeñaAlejandra Bravo
Aug 20, 2004·Journal of Bacteriology·Qi XuRaphael Lamed

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