A Generalist Lifestyle Allows Rare Gardnerella spp. to Persist at Low Levels in the Vaginal Microbiome.

Microbial Ecology
S. KhanJanet E Hill

Abstract

Gardnerella spp. are considered a hallmark of bacterial vaginosis, a dysbiosis of the vaginal microbiome. There are four cpn60 sequence-based subgroups within the genus (A, B, C and D), and thirteen genome species have been defined recently. Gardnerella spp. co-occur in the vaginal microbiome with varying abundance, and these patterns are shaped by a resource-dependent, exploitative competition, which affects the growth rate of subgroups A, B and C negatively. The growth rate of rarely abundant subgroup D, however, increases with the increasing number of competitors, negatively affecting the growth rate of others. We hypothesized that a nutritional generalist lifestyle and minimal niche overlap with the other more abundant Gardnerella spp. facilitate the maintenance of subgroup D in the vaginal microbiome through negative frequency-dependent selection. Using 40 whole-genome sequences from isolates representing all four subgroups, we found that they could be distinguished based on the content of their predicted proteomes. Proteins associated with carbohydrate and amino acid uptake and metabolism were significant contributors to the separation of subgroups. Subgroup D isolates had significantly more of their proteins assigned to ...Continue Reading

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Citations

Dec 29, 2020·Infection and Immunity·Shakya P KurukulasuriyaJanet E Hill

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Datasets Mentioned

BETA
PRJNA55487

Methods Mentioned

BETA
PCR

Software Mentioned

UPGMA
WebMEGA
SIMPER
Trimmomatic
DendroUPGMA
vegan
SOAPdenovo2
PERMANOVA
SPAdes
RStudio

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