A generic magnetic microsphere platform with "clickable" ligands for purification and immobilization of targeted proteins

ACS Applied Materials & Interfaces
Jin ZhengWuli Yang

Abstract

While much effort has been made to prepare magnetic microspheres (MMs) with surface moieties that bind to affinity tags or fusion partners of interest in the recombinant proteins, it remains a challenge to develop a generic platform that is capable of incorporating a variety of capture ligands by a simple chemistry. Herein, we developed core-shell structured magnetic microspheres with a high magnetic susceptibility and a low nonspecific protein adsorption. Surface functionalization of these MMs with azide groups facilitates covalent attachment of alkynylated ligands on their surfaces by "click" chemistry and creates a versatile platform for selective purification and immobilization of recombinant proteins carrying corresponding affinity tags. The general applicability of the approach was demonstrated in incorporating four widely used affinity ligands with different reactive groups (-CHO, -SH, -COOH, and -NH2) onto the MMs platform for purification and immobilization of targeted proteins. The azide-functionalized MMs would be applicable for a variety of ligands and substrates that are amenable to alkynylation modification.

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Jan 11, 2014·ACS Applied Materials & Interfaces·Jin ZhengWuli Yang
Apr 29, 2014·ACS Applied Materials & Interfaces·Yuting ZhangChangchun Wang

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Citations

Feb 23, 2017·Journal of the American Chemical Society·Xirui GongJ Fraser Stoddart
Nov 28, 2019·ChemMedChem·Ramon van der VlagAnna K H Hirsch

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