PMID: 9438203Jan 23, 1998Paper

A generic sequencing based typing approach for the identification of HLA-A diversity

Human Immunology
S A ScheltingaM G Tilanus

Abstract

Sequencing Based Typing (SBT) is a generic approach for the identification of HLA-A polymorphism. This approach includes the high resolution typing of the HLA-A broad reacting groups, HLA-A subtypes and will identify new alleles directly. The SBT approach described here uses a locus specific amplification of DNA from exon 1 to exon 5. The resulting 2,022 bp PCR product serves as a template for the subsequent sequencing reactions. Amplification is followed by direct sequencing of exons 2, 3 and 4 in both orientations with fluorescently labeled primers to define all polymorphic positions leading to a high resolution typing result. In this study the sequence of exons 2 and 3 of a panel of 49 cell lines was determined. In addition, the exon 4 region of 35 cell lines was also sequenced to evaluate the exon 4 polymorphism. The HLA-A type of most of the cells could be identified by sequencing only exons 2 and 3. However, the sequence of exon 4 was required to discriminate A*0201 from A*0209 and A*0207 from A*0215N. In this panel, an identical new "HLA-A*0103" was identified in two Caucasian samples.

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Citations

Jun 7, 2005·Human Immunology·Wendy T N SwelsenElla M van den Berg-Loonen
Feb 22, 2001·Human Immunology·G A PolandS B Moore
Oct 6, 2005·Bone Marrow Transplantation·G La NasaL Contu
Mar 9, 1999·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·A M Little, J A Madrigal
Nov 5, 1999·Vox Sanguinis·J G BodmerP I Terasaki
Dec 12, 2001·Transplantation·R W CollinsR W Vaughan
Jul 22, 1998·Tissue Antigens·S A ScheltingaM G Tilanus
Apr 15, 1999·Tissue Antigens·S PozziG B Ferrara
May 31, 2001·Tissue Antigens·S NesciG Lucarelli
Jul 30, 2002·Tissue Antigens·E H RozemullerM G J Tilanus
Mar 7, 2000·Tissue Antigens·M BunceK I Welsh
Mar 7, 2000·Tissue Antigens·S A ScheltingaS Wu
Oct 4, 2015·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Claudio G BrunsteinJohn E Wagner
Dec 13, 2005·Annals of the New York Academy of Sciences·Giorgio La NasaFranco Locatelli
Jun 26, 2003·Tissue Antigens·W T N SwelsenE M van den Berg-Loonen
Dec 12, 2007·Tissue Antigens·E M ThompsonL Tidey
Jan 21, 2014·Tissue Antigens·D WangH Zou
Jun 4, 2005·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·E C CastelliJ L V de Camargo
Mar 2, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Claudia de LallaGiulia Casorati
May 25, 2002·Tissue Antigens·David M SmillieP P J Dunn
May 13, 1999·Tissue Antigens·J G BodmerP I Terasaki

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