A genetic and physical study of the interdomain linker of E. Coli AraC protein--a trans-subunit communication pathway

Proteins
Fabiana MalagaRobert Schleif

Abstract

Genetic experiments with full length AraC and biophysical experiments with its dimerization domain plus linker suggest that arabinose binding to the dimerization domain changes the properties of the inter-domain linker which connects the dimerization domain to the DNA binding domain via interactions that do not depend on the DNA binding domain. Normal AraC function was found to tolerate considerable linker sequence alteration excepting proline substitutions. The proline substitutions partially activate transcription even in the absence of arabinose and hint that a structural shift between helix and coil may be involved. To permit fluorescence anisotropy measurements that could detect arabinose-dependent dynamic differences in the linkers, IAEDANS was conjugated to a cysteine residue substituted at the end of the linker of dimerization domain. Arabinose, but not other sugars, decreased the steady-state anisotropy, indicating either an increase in mobility and/or an increase in the fluorescence lifetime of the IAEDANS. Time-resolved fluorescence measurements showed that the arabinose-induced anisotropy decrease did not result from an increase in the excited-state lifetime. Hence arabinose-induced decreases in anisotropy appear to...Continue Reading

References

Oct 26, 1990·Science·R B Lobell, R F Schleif
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D H Lee, R F Schleif
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K MartinR F Schleif
Oct 6, 1971·Nature: New Biology·J Greenblatt, R Schleif
Apr 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·E EnglesbergF Meronk
Oct 1, 1965·Journal of Bacteriology·E EnglesbergN Lee
Nov 25, 1984·Journal of Molecular Biology·S HahnR Schleif
Dec 1, 1996·Journal of Bacteriology·R J Eustance, R F Schleif
Apr 18, 1997·Science·S M SoissonC Wolberger
Jun 20, 1998·Journal of Molecular Biology·R R Seabold, R F Schleif
Jun 20, 1998·Journal of Molecular Biology·B SaviolaR F Schleif
Jun 27, 1998·Journal of Molecular Biology·P OrthW Hinrichs
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·S RheeD R Davies
Aug 31, 2000·Nature Structural Biology·S D Bell, S P Jackson
Feb 17, 2001·Current Opinion in Structural Biology·C E Bell, M Lewis
Apr 5, 2001·Journal of Molecular Biology·M Wu, R Schleif
Jul 31, 2001·Analytical Biochemistry·M Ghosh, R F Schleif
Dec 18, 2002·Journal of Bacteriology·Don WalthersLinda J Kenney
Apr 10, 2003·Journal of Molecular Biology·Jennifer J RossRobert Schleif
Jun 30, 2004·Journal of Molecular Biology·Andrew TimmesRobert Schleif
Aug 18, 2004·Journal of Molecular Biology·Birgitte H Kallipolitis, Poul Valentin-Hansen
Oct 31, 2006·Journal of Bacteriology·Ana KolinSusan M Egan
Dec 19, 2006·Proteins·John E WeldonRobert F Schleif
Jun 19, 2008·Proteins·Michael E RodgersRobert Schleif
Feb 17, 2009·Journal of Bacteriology·Stephanie DirlaRobert Schleif
Mar 25, 2009·Protein Science : a Publication of the Protein Society·Jennifer E SeedorffRobert Schleif
Apr 11, 2009·Proceedings of the National Academy of Sciences of the United States of America·Nataliya PopovychCharalampos G Kalodimos
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hitesh SharmaThomas A Steitz
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Michael J LowdenF Jon Kull
Jul 19, 2011·Journal of Molecular Biology·Divya Prasanna KumarQinglian Liu
Aug 16, 2011·Journal of Bacteriology·Jennifer Seedorff, Robert Schleif
Mar 3, 2012·Proteins·Stephanie Dirla Cole, Robert Schleif
Feb 5, 2013·Nature Cell Biology·Daniel EschHans R Schöler

❮ Previous
Next ❯

Citations

Nov 8, 2017·Proceedings of the National Academy of Sciences of the United States of America·Yunfeng GaoLinda J Kenney
Apr 13, 2021·Frontiers in Genetics·Nosheen Afzal QureshiSyed Babar Jamal
Apr 11, 2021·FEMS Microbiology Reviews·Daniel Cortés-AvalosJ Antonio Ibarra
Jun 15, 2019·Biochemistry·Matthew J Brown, Robert Schleif

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.