A genetic approach for controlling the binding and orientation of proteins on nanoparticles

Langmuir : the ACS Journal of Surfaces and Colloids
Atanu SenguptaFrançois Baneyx

Abstract

Although silver nanoparticles are excellent surface enhancers for Raman spectroscopy, their use to probe the conformation of large proteins at interfaces has been complicated by the fact that many polypeptides adsorb weakly or with a random orientation to colloidal silver. To address these limitations, we sought to increase binding affinity and control protein orientation by fusing a silver-binding dodecapeptide termed Ag4 to the C-terminus of maltose-binding protein (MBP), a well-characterized model protein with little intrinsic silver binding affinity. Quartz crystal microbalance measurements conducted with the MBP-Ag4 fusion protein revealed that its affinity for silver (Kd approximately 180 nM) was at least 1 order of magnitude higher than a control protein, MBP2, containing a non-silver-specific C-terminal extension. Under our experimental conditions, MBP-Ag4 SERS spectra exhibited 2-4 fold higher signal-to-background relative to MPB2 and contained a number of amino acid-assigned vibrational modes that were either weak or absent in control experiments performed with MBP2. Changes in amino acid-assigned peaks before and after MBP-Ag4 bound maltose were used to assess protein orientation on the surface of silver nanoparticle...Continue Reading

References

Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R B MikkelsenD F Wallach
Mar 28, 1997·Journal of Molecular Biology·C Manoil, J Bailey
Dec 28, 1999·Methods : a Companion to Methods in Enzymology·C Manoil, B Traxler
Jul 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Marcus FehrSylvie Lalonde
Mar 6, 2003·Nature Materials·Rajesh R NaikMorley O Stone
Sep 3, 2003·Nature Materials·Mehmet SarikayaFrançois Baneyx
Apr 23, 2004·Current Opinion in Structural Biology·Jeffrey J Gray
Dec 25, 2004·Biophysical Chemistry·Ines DelfinoSalvatore Cannistraro
Aug 18, 2005·Applied Spectroscopy·Atanu SenguptaE James Davis
Oct 11, 2005·Advances in Colloid and Interface Science·R F ArocaJ V Garcia-Ramos
Nov 3, 2005·Journal of the American Chemical Society·Haixia DaiDaniel T Schwartz
Jan 31, 2006·IEE Proceedings. Nanobiotechnology·D A StuartR P Van Duyne
Oct 28, 2006·Annual Review of Physical Chemistry·Katherine A Willets, Richard P Van Duyne
Jul 10, 2007·Current Opinion in Biotechnology·François Baneyx, Daniel T Schwartz

❮ Previous
Next ❯

Citations

Apr 23, 2009·Analytical and Bioanalytical Chemistry·Mohammad Kamal HossainYukihiro Ozaki
Mar 31, 2012·ACS Applied Materials & Interfaces·M HnilovaC Tamerler
Mar 16, 2013·Chemical Reviews·Sonja EckhardtKatharina M Fromm
Mar 12, 2010·Journal of the American Chemical Society·Weibin ZhouFrançois Baneyx
May 14, 2010·Journal of the Royal Society, Interface·Sara Abalde-CelaLuis M Liz-Marzán
Jan 31, 2012·Applied and Environmental Microbiology·Ruth Hall SedlakBeth Traxler
Jul 15, 2011·Nanoscale Research Letters·Yan ZhangShouwu Guo
Jun 28, 2014·Applied Biochemistry and Biotechnology·Andrew CareAnwar Sunna
Mar 5, 2013·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Rayk Hassert, Annette G Beck-Sickinger
Sep 10, 2010·Chembiochem : a European Journal of Chemical Biology·Ruth Hall SedlakBeth Traxler
Dec 16, 2011·Biotechnology and Bioengineering·Marketa HnilovaCandan Tamerler
Mar 1, 2012·Biotechnology and Bioengineering·Sathana KitayapornFrançois Baneyx
Feb 7, 2013·Biotechnology Journal·Sibel CetinelCandan Tamerler
Mar 23, 2015·Trends in Biotechnology·Andrew CareAnwar Sunna
Nov 17, 2012·Journal of Immunological Methods·Mateusz S Szymanski, Robert A Porter
Aug 6, 2016·Physical Chemistry Chemical Physics : PCCP·A GladytzB Abel
Feb 11, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Urartu Ozgur Safak Seker, Hilmi Volkan Demir
Apr 2, 2010·ACS Applied Materials & Interfaces·Adam A SkeltonTiffany R Walsh
Apr 20, 2021·Biomacromolecules·Nicolò AlvisiRenko de Vries
Apr 15, 2019·JOM : the Journal of the Minerals, Metals & Materials Society·Esra Yuca, Candan Tamerler
Mar 21, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Brandon L CoyleFrançois Baneyx
May 3, 2014·The Journal of Physical Chemistry. B·Partha P KunduChandrabhas Narayana
Dec 19, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Xiaoxing ChenPing Wang
Aug 23, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Neus FeliuRamon Alvarez-Puebla

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.