A genetic approach to detect muscle protein interactions in vivo

Trends in Cardiovascular Medicine
A FerrúsA Hernández-Hernández

Abstract

An organism is required to identify biologically relevant protein interactions. We propose Drosophila and its indirect flight muscles as a suitable experimental system for genetic screenings for muscle protein interactions. The first attempt focused on troponin I (TnI) in view of the key role in thin filament regulation that this protein performs. Suppressors of a defined Tn I allele have been isolated as mutations in the heavy chain of myosin (MhC). This unsuspected functional interaction between TnI and MhC serves to illustrate one of the benefits of the approach. Four of the suppressors identified to date reside in the MhC head, around the actin-binding site and near the lips of the pocket where ATP is hydrolyzed. Two other suppressors correspond to a second site mutation in TnI and a mutation in the conserved region of Tropomyosin II (TmII), respectively. All the identified suppressors are mutations in constituents of the sarcomere, and most of them are structurally similar to human mutations causing familial hypertrophic cardiomyopathy (FHC). At least seven sarcomere proteins can lead to FHC and, consequently, the disease is heterogeneous and difficult to diagnose. In addition, putative natural suppressors may help obscure...Continue Reading

References

Jan 1, 1991·Journal of Cell Science. Supplement·J SparrowD White
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R F SiemankowskiH D White
Mar 1, 1997·Journal of the American College of Cardiology·J C MoolmanH Watkins
Oct 23, 1997·Biophysical Journal·D Cabral-LillyC Cohen
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·D G VassylyevY Maéda
Jul 24, 1998·Molecular and Cellular Neurosciences·S M CatalanoA Chédotal
Mar 23, 1999·The Journal of Cell Biology·W A KronertS I Bernstein
Jun 4, 1999·Seminars in Cell & Developmental Biology·M Frasch
Jun 4, 1999·Seminars in Cell & Developmental Biology·S M Evans
Oct 8, 1999·Molecular Biology of the Cell·D M HelfmanA D Bershadsky
Dec 11, 1999·Journal of Molecular and Cellular Cardiology·P JääskeläinenJ Kuusisto
Jan 1, 2000·Cardiovascular Research·C S RedwoodH Watkins
May 22, 2001·Molecular Biology of the Cell·B NaimiJ C Sparrow
Dec 14, 2002·Journal of the Neurological Sciences·C G BönnemannR Schröder
Mar 26, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Elisabeth EhlerJean-Claude Perriard
Jan 1, 1995·Trends in Cardiovascular Medicine·R Bodmer
Apr 18, 2014·Journal of Muscle Research and Cell Motility·Sherwin S Lehrer, Michael A Geeves

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Citations

Mar 31, 2004·The Journal of Cell Biology·Enrico S Montana, J Troy Littleton
Jul 1, 2005·Physiological Reviews·Scott L Hooper, Jeffrey B Thuma

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