A genetic basis for molecular asymmetry at vertebrate electrical synapses

ELife
Adam C MillerCecilia B Moens

Abstract

Neural network function is based upon the patterns and types of connections made between neurons. Neuronal synapses are adhesions specialized for communication and they come in two types, chemical and electrical. Communication at chemical synapses occurs via neurotransmitter release whereas electrical synapses utilize gap junctions for direct ionic and metabolic coupling. Electrical synapses are often viewed as symmetrical structures, with the same components making both sides of the gap junction. By contrast, we show that a broad set of electrical synapses in zebrafish, Danio rerio, require two gap-junction-forming Connexins for formation and function. We find that one Connexin functions presynaptically while the other functions postsynaptically in forming the channels. We also show that these synapses are required for the speed and coordination of escape responses. Our data identify a genetic basis for molecular asymmetry at vertebrate electrical synapses and show they are required for appropriate behavioral performance.

References

Jan 1, 1991·Brain, Behavior and Evolution·J R Fetcho
Jun 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·E Raviola, N B Gilula
Mar 1, 1969·The Journal of Cell Biology·M W Brightman, T S Reese
May 1, 1981·The Journal of Comparative Neurology·C B KimmelR J Kimmel
Jul 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C B KimmelT F Schilling
Jun 3, 1999·Gene·K D CurtinR J Wyman
Jun 5, 2002·Genome Research·W James KentDavid Haussler
Mar 23, 2004·Biochimica Et Biophysica Acta·Sheriar G HormuzdiRoberto Bruzzone
Apr 20, 2004·Cardiovascular Research·Goran Söhl, Klaus Willecke
Jun 18, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·John O'BrienStephen L Mills
Jun 26, 2004·Annual Review of Neuroscience·Barry W Connors, Michael A Long
Sep 15, 2004·Trends in Genetics : TIG·John PostlethwaitYi-Lin Yan
Dec 2, 2004·Brain Research. Brain Research Reviews·Alberto E PeredaMichael V L Bennett
Mar 2, 2005·Nature Reviews. Neuroscience·Goran SöhlKlaus Willecke
Jun 1, 2005·Biochimica Et Biophysica Acta·Pauline Phelan
Jun 1, 2005·Neuron·Jason M ChristieGary L Westbrook
Oct 31, 2006·Results and Problems in Cell Differentiation·Carola Meier, Rolf Dermietzel
Mar 7, 2007·Biological Chemistry·Véronique Cruciani, Svein-Ole Mikalsen
May 4, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Harold A Burgess, Michael Granato
Jul 24, 2008·Cell Communication & Adhesion·Georg ZoidlRolf Dermietzel
Oct 7, 2008·Briefings in Functional Genomics & Proteomics·Harold A Burgess, Michael Granato
May 29, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Chie SatouShin-ichi Higashijima
Jul 2, 2009·Journal of Visualized Experiments : JoVE·Charline WalkerCecilia Moens
Jul 21, 2009·Journal of Visualized Experiments : JoVE·Hilary A KempCecilia Moens
Dec 4, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Hongyan LiJohn O'Brien
Jan 13, 2010·Cold Spring Harbor Perspectives in Biology·Daniel A Goodenough, David L Paul
Apr 29, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Kevin AllenHannah Monyer
Aug 31, 2011·Proceedings of the National Academy of Sciences of the United States of America·Marc A WolmanMichael Granato
Sep 20, 2011·Methods in Cell Biology·Ross N W KettleboroughDerek L Stemple
Oct 25, 2011·Science·Julie S HaasCarole E Landisman
Jan 10, 2012·Nature Protocols·Neville E SanjanaFeng Zhang
Jan 24, 2012·Neuroscience Letters·Jiook ChaIn-Beom Kim
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Aug 14, 2013·The Journal of Comparative Neurology·Tara C Carlisle, Angeles B Ribera
Sep 10, 2013·Neuron·John E RashAlberto E Pereda
Mar 13, 2014·Nature Reviews. Neuroscience·Alberto E Pereda
May 23, 2014·Journal of Neurophysiology·Kathryn M TaborHarold A Burgess
Aug 20, 2014·Nature Communications·Nicolás Palacios-PradoFeliksas F Bukauskas
Nov 2, 2014·Frontiers in Cellular Neuroscience·Nicolás Palacios-PradoAlberto E Pereda

❮ Previous
Next ❯

Citations

Aug 2, 2018·Journal of Neurophysiology·Shaista Jabeen, Vatsala Thirumalai
Aug 25, 2020·Science Advances·Nange JinChristophe P Ribelayga
Aug 17, 2019·Journal of Molecular Biology·Gabriela Da Silva Xavier, Guy A Rutter
Sep 17, 2020·Science Advances·Nange JinChristophe P Ribelayga
Apr 29, 2021·ELife·Abagael M LasseigneAdam C Miller
May 1, 2021·International Journal of Molecular Sciences·Georg R Zoidl, David C Spray
May 4, 2021·PLoS Computational Biology·JaeAnn M DwuletRichard K P Benninger
Jun 5, 2021·Communications Biology·Wim H QuintAdriana I Iglesias
Aug 21, 2021·Pediatric Neurology·Harvey B Sarnat

❮ Previous
Next ❯

Methods Mentioned

BETA
electron microscopy
RNA-seq
transgenic
Hi-Seq
genotyping

Software Mentioned

Illustrator
Adobe
Ensembl Genome Browser
Fiji
CoLos
Photoshop
FLOTE

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
L VenanceH Monyer
Nature Reviews. Neuroscience
Goran SöhlKlaus Willecke
Brain Research. Brain Research Reviews
R RozentalDavid C Spray
© 2022 Meta ULC. All rights reserved