A genetic locus targeted to the nuclear periphery in living cells maintains its transcriptional competence

The Journal of Cell Biology
R Ileng Kumaran, David L Spector

Abstract

The peripheral nuclear lamina, which is largely but not entirely associated with inactive chromatin, is considered to be an important determinant of nuclear structure and gene expression. We present here an inducible system to target a genetic locus to the nuclear lamina in living mammalian cells. Using three-dimensional time-lapse microscopy, we determined that targeting of the locus requires passage through mitosis. Once targeted, the locus remains anchored to the nuclear periphery in interphase as well as in daughter cells after passage through a subsequent mitosis. Upon transcriptional induction, components of the gene expression machinery are recruited to the targeted locus, and we visualized nascent transcripts at the nuclear periphery. The kinetics of transcriptional induction at the nuclear lamina is similar to that observed at an internal nuclear region. This new cell system provides a powerful approach to study the dynamics of gene function at the nuclear periphery in living cells.

References

Sep 18, 1992·Cell·M E LudérusR van Driel
Jan 1, 1993·Annual Review of Cell Biology·D L Spector
Jun 15, 1999·The Journal of Cell Biology·J A CroftW A Bickmore
Jun 29, 1999·The Journal of Cell Biology·T TumbarA S Belmont
Oct 6, 1999·The Journal of Cell Biology·P J VerschureR van Driel
Dec 7, 2000·Molecular Cell·T I GerasimovaV G Corces
Jan 9, 2001·Nature Cell Biology·T TsukamotoD L Spector
Apr 3, 2001·Nature Reviews. Genetics·T Cremer, C Cremer
May 18, 2001·Trends in Cell Biology·A S Belmont
Nov 28, 2001·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·M CremerT Cremer
Mar 6, 2002·Genes & Development·Robert D GoldmanTimothy P Spann
Mar 8, 2002·Current Biology : CB·Wallace F Marshall
Mar 23, 2002·Current Biology : CB·Jonathan R ChubbWendy A Bickmore
Jun 14, 2002·Cell·Kojiro IshiiUlrich K Laemmli
Nov 26, 2002·EMBO Reports·Matthias BeckerGordon L Hager
Nov 26, 2002·Science·Miroslav DundrTom Misteli
Dec 11, 2002·The Journal of Cell Biology·Hiroshi KimuraPeter R Cook
Jul 31, 2003·Trends in Cell Biology·Luis A ParadaTom Misteli
Oct 7, 2003·Annual Review of Biochemistry·David L Spector
Mar 10, 2004·Cell·Susan M JanickiDavid L Spector
Jun 19, 2004·Science·Yaron Shav-TalRobert H Singer
Jul 9, 2004·Cytogenetic and Genome Research·S H KimT Misteli
Sep 15, 2004·The Journal of Cell Biology·Daniele ZinkDirk Schindelhauer
Sep 30, 2004·PLoS Biology·Jason H Brickner, Peter Walter
Apr 16, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Tom Misteli
Aug 30, 2005·Trends in Biochemical Sciences·Eric C Schirmer, Larry Gerace
Oct 11, 2005·Annual Review of Cell and Developmental Biology·Martin W HetzerIain W Mattaj
Dec 24, 2005·Journal of Cell Science·Ruth R E WilliamsAmanda G Fisher
Apr 8, 2006·Genes & Development·Hansol LeeCory Abate-Shen
Apr 25, 2006·Current Biology : CB·Chien-Hui ChuangAndrew S Belmont

❮ Previous
Next ❯

Citations

Jul 4, 2008·Chromosoma·Claudia HeppergerSteffen Dietzel
Jul 25, 2008·Chromosoma·Karen J MeaburnJoanna M Bridger
Oct 15, 2008·Chromosoma·Elzo de Wit, Bas van Steensel
Sep 4, 2009·Chromosoma·Dave A Pai, David R Engelke
Jun 23, 2011·Chromosoma·Romain SolinhacMartine Yerle-Bouissou
Oct 24, 2012·Chromosoma·K Laurence JostM Cristina Cardoso
Dec 17, 2009·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Benjamin D PopeDavid M Gilbert
Feb 10, 2010·Journal of Mammary Gland Biology and Neoplasia·Clémence KressMonique Rijnkels
Jun 12, 2013·Trends in Biochemical Sciences·Benjamin D TowbinSusan M Gasser
Feb 16, 2011·Cell Research·Shigeki NagaiSusan M Gasser
Oct 15, 2010·Nature Biotechnology·Bas van Steensel, Job Dekker
Dec 21, 2010·Nature Cell Biology·Yuntao S MaoDavid L Spector
Apr 24, 2010·Nature Reviews. Molecular Cell Biology·Karim Mekhail, Danesh Moazed
Dec 6, 2012·Nature Reviews. Molecular Cell Biology·Brian Burke, Colin L Stewart
Mar 7, 2013·Nature Structural & Molecular Biology·Giacomo Cavalli, Tom Misteli
Feb 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Y Y ShevelyovD I Nurminsky
Nov 6, 2009·Proceedings of the National Academy of Sciences of the United States of America·Tomoki YokochiDavid M Gilbert
Feb 11, 2012·Molecular Biology of the Cell·Erin M GreenKarsten Weis
Jan 25, 2011·Briefings in Functional Genomics·Cameron S OsborneAlice N C Young
Aug 21, 2013·Nucleic Acids Research·Zhong ChenSteffen E Meiler
Sep 10, 2010·Cold Spring Harbor Perspectives in Biology·Thomas DechatRobert D Goldman
May 21, 2010·Cold Spring Harbor Perspectives in Biology·Carmelo FerraiAna Pombo
Jul 30, 2010·Cold Spring Harbor Perspectives in Biology·Lyubomira Chakalova, Peter Fraser
Mar 20, 2010·Cold Spring Harbor Perspectives in Biology·Thomas Cremer, Marion Cremer
Dec 14, 2011·Genes & Development·Takeshi ShimiRobert D Goldman
Jan 5, 2012·Genes & Development·Elzo de Wit, Wouter de Laat
Sep 29, 2011·Plant Physiology·Joanna BorucIris Meier
Nov 26, 2010·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Michèle Amouyal
Jul 10, 2013·Annual Review of Cell and Developmental Biology·Joan C Ritland PolitzMark Groudine
Jul 24, 2013·Annual Review of Genomics and Human Genetics·Wendy A Bickmore
Apr 9, 2010·Annual Review of Biochemistry·Hisao MasaiMasako Oda
May 7, 2009·Annual Review of Biophysics·Xavier DarzacqRobert H Singer
May 14, 2010·Annual Review of Biophysics·Michael R Hübner, David L Spector

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
gene array
PCR

Software Mentioned

OpenLab
Improvision
QuickTime Pro
SoftWoRx
Illustrator
Photoshop

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.