A genetic screen to isolate Toxoplasma gondii host-cell egress mutants.

Journal of Visualized Experiments : JoVE
Bradley I Coleman, Marc-Jan Gubbels

Abstract

The widespread, obligate intracellular, protozoan parasite Toxoplasma gondii causes opportunistic disease in immuno-compromised patients and causes birth defects upon congenital infection. The lytic replication cycle is characterized by three stages: 1. active invasion of a nucleated host cell; 2. replication inside the host cell; 3. active egress from the host cell. The mechanism of egress is increasingly being appreciated as a unique, highly regulated process, which is still poorly understood at the molecular level. The signaling pathways underlying egress have been characterized through the use of pharmacological agents acting on different aspects of the pathways. As such, several independent triggers of egress have been identified which all converge on the release of intracellular Ca(2+), a signal that is also critical for host cell invasion. This insight informed a candidate gene approach which led to the identification of plant like calcium dependent protein kinase (CDPK) involved in egress. In addition, several recent breakthroughs in understanding egress have been made using (chemical) genetic approaches. To combine the wealth of pharmacological information with the increasing genetic accessibility of Toxoplasma we rece...Continue Reading

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Citations

Mar 22, 2013·Biochemical Society Transactions·Leanne M Smith, Robin C May
Dec 7, 2016·Antimicrobial Agents and Chemotherapy·P Holland AldayJ Stone Doggett
Aug 26, 2015·Antimicrobial Agents and Chemotherapy·Imaan BenmerzougaWilliam J Sullivan
Aug 17, 2018·Pathogens·Lucio Ayres Caldas, Wanderley de Souza

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