A genetic selection for Neurospora crassa mutants altered in their light regulation of transcription.

Genetics
Laura Navarro-SampedroLuis M Corrochano

Abstract

Transcription of the Neurospora crassa gene con-10 is induced during conidiation and following exposure of vegetative mycelia to light, but light activation is transient due to photoadaptation. We describe mutational analyses of photoadaptation using a N. crassa strain bearing a translational fusion of con-10, including its regulatory region, to a selectable bacterial gene conferring hygromycin resistance (hph). Growth of this strain was sensitive to hygromycin, upon continuous culture in the light. Five mutants were isolated that were resistant to hygromycin when cultured under constant light. Three mutant strains displayed elevated, sustained accumulation of con-10::hph mRNA during continued light exposure, suggesting that they bear mutations that reduce or eliminate the presumed light-dependent repression mechanism that blocks con-10 transcription upon prolonged illumination. These mutations altered photoadaptation for only a specific group of genes (con-10 and con-6), suggesting that regulation of photoadaptation is relatively gene specific. The mutations increased light-dependent mRNA accumulation for genes al-1, al-2, and al-3, each required for carotenoid biosynthesis, resulting in a threefold increase in carotenoid accu...Continue Reading

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Citations

Apr 22, 2009·Proceedings of the National Academy of Sciences of the United States of America·Catalina SanzArturo P Eslava
Aug 25, 2010·Proceedings of the National Academy of Sciences of the United States of America·Chen-Hui ChenJennifer J Loros
Feb 19, 2013·Applied and Environmental Microbiology·Marta CastrilloJavier Avalos
Aug 26, 2014·Fungal Genetics and Biology : FG & B·Marta Castrillo, Javier Avalos
Oct 7, 2014·Current Genetics·Javier Avalos, M Carmen Limón
Jul 20, 2010·Fungal Genetics and Biology : FG & B·Chen-Hui ChenJennifer J Loros
Aug 28, 2019·Annual Review of Genetics·Luis M Corrochano
Mar 24, 2018·Applied Microbiology and Biotechnology·Kevin K FullerJennifer J Loros

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