A genetic strategy for combined screening and localized imaging of breast cancer.

Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging
Jason M WarramKurt R Zinn

Abstract

Improvements are needed for the early detection of breast cancer, as current imaging methods lack sensitivity to detect small tumors and assess their disease phenotype. To address this issue, the dual reporter adenoviral vector (Ad5/3-Id1-SEAP-Id1-mCherry) was produced with a cancer-specific Id1 promoter driving expression of a blood-based screening reporter (secreted embryonic alkaline phosphatase, SEAP) and a fluorescent imaging reporter (mCherry). This diagnostic system was assessed for its screening potential on breast cancer cell lines of various aggressive phenotypes. Reporter expression was measured and correlated with promoter level expression using Western blot. Adenovirus receptor expression was normalized against reporter expression with luciferase infectivity assays. Ad5/3-Id1-SEAP-Id1-mCherry infected MDA-MB-231 cells combined with uninfected cells were implanted into the mammary fat pad of athymic nude mice to recapitulate low-dose tumor delivery. Id1 driven SEAP expression and mCherry imaging were monitored to validate diagnostic sensitivity and efficacy. Infected breast cancer cell lines displayed SEAP levels in the media that were 10-fold above background by 2 days after infection. Ad5/3-Id1-SEAP-Id1-mCherry in...Continue Reading

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Citations

Feb 26, 2015·Proceedings of the National Academy of Sciences of the United States of America·John A RonaldSanjiv S Gambhir
Feb 22, 2012·The International Journal of Biochemistry & Cell Biology·Hyo-eun C Bhang, Martin G Pomper
Sep 8, 2021·Nature Reviews. Cancer·Gabriel A KwongSangeeta N Bhatia

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