A genetic strategy for stochastic gene activation with regulated sparseness (STARS).

PloS One
Sheng-Zhi WangLi I Zhang

Abstract

It remains a challenge to establish a straightforward genetic approach for controlling the probability of gene activation or knockout at a desired level. Here, we developed a method termed STARS: stochastic gene activation with genetically regulated sparseness. The stochastic expression was achieved by two cross-linked, mutually-exclusive Cre-mediated recombinations. The stochastic level was further controlled by regulating Cre/lox reaction kinetics through varying the intrachromosomal distance between the lox sites mediating one of the recombinations. In mammalian cell lines stably transfected with a single copy of different STARS transgenes, the activation/knockout of reporter genes was specifically controlled to occur in from 5% to 50% of the cell population. STARS can potentially provide a convenient way for genetic labeling as well as gene expression/knockout in a population of cells with a desired sparseness level.

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Citations

Sep 4, 2010·Genetics·Kirsten A TurloM Luisa Iruela-Arispe
Apr 28, 2018·Biotechnology Journal·Xue WangMasamichi Kamihira
Aug 31, 2017·Nature Reviews. Genetics·Raj Chari, George M Church
Jul 6, 2020·Scientific Reports·Addeli Bez Batti AngulskiJoseph M Metzger

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Methods Mentioned

BETA
transgenic
transfection
FACS
transfections

Software Mentioned

Brainbow
STARS

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