A genetic suppressor of two dominant temperature-sensitive lethal proteasome mutants of Drosophila melanogaster is itself a mutated proteasome subunit gene.

Genetics
Peter J NeuburgerJ M Belote

Abstract

Two dominant temperature-sensitive (DTS) lethal mutants of Drosophila melanogaster are Pros26(1) and Prosbeta2(1), previously known as DTS5 and DTS7. Heterozygotes for either mutant die as pupae when raised at 29 degrees , but are normally viable and fertile at 25 degrees . Previous studies have identified these as missense mutations in the genes encoding the beta6 and beta2 subunits of the 20S proteasome, respectively. In an effort to isolate additional proteasome-related mutants a screen for dominant suppressors of Pros26(1) was carried out, resulting in the identification of Pros25(SuDTS) [originally called Su(DTS)], a missense mutation in the gene encoding the 20S proteasome alpha2 subunit. Pros25(SuDTS) acts in a dominant manner to rescue both Pros26(1) and Prosbeta2(1) from their DTS lethal phenotypes. Using an in vivo protein degradation assay it was shown that this suppression occurs by counteracting the dominant-negative effect of the DTS mutant on proteasome activity. Pros25(SuDTS) is a recessive polyphasic lethal at ambient temperatures. The effects of these mutants on larval neuroblast mitosis were also examined. While Prosbeta2(1) shows a modest increase in the number of defective mitotic figures, there were no def...Continue Reading

References

Apr 1, 1992·Archives of Biochemistry and Biophysics·M E PereiraS Wilk
Apr 1, 1989·Genes & Development·M Gatti, B S Baker
Apr 1, 1989·Genetics·P NovickD Botstein
Jan 1, 1987·Annual Review of Genetics·T C HuffakerD Botstein
Aug 19, 1993·Biochimica Et Biophysica Acta·A SeeligP M Kloetzel
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K J Saville, J M Belote
Mar 1, 1996·European Journal of Biochemistry·M A KaniaA L Goldberg
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·R Y Hampton, H Bhakta
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·F Schweisguth
May 3, 2000·The Journal of Cell Biology·C S Wesley, L Saez
Jul 25, 2000·Seminars in Cell & Developmental Biology·Y Ye, M E Fortini
Nov 4, 2000·Nature Structural Biology·M GrollD Finley
Jun 19, 2001·Medicinal Research Reviews·J MyungC M Crews
Mar 28, 2002·Physiological Reviews·Michael H Glickman, Aaron Ciechanover
May 17, 2002·Structure·Masaki UnnoTomitake Tsukihara
Sep 27, 2002·Genesis : the Journal of Genetics and Development·John M Belote, Eric Fortier
Oct 24, 2002·Human Molecular Genetics·H Y Edwin ChanNancy M Bonini
Oct 29, 2002·Current Biology : CB·Ranjiv S KhushBruno Lemaitre
Nov 20, 2003·Nature Reviews. Molecular Cell Biology·Steven I Reed
Dec 12, 2003·Genetics·Joshua M Shulman, Mel B Feany
Apr 8, 2004·Developmental Cell·Josh Friedman, Ding Xue
Jun 26, 2004·Annual Review of Neuroscience·Aaron DiAntonio, Linda Hicke
Dec 2, 2004·Biochimica Et Biophysica Acta·Peter-M Kloetzel
Dec 21, 2004·Nucleic Acids Research·Rachel A DrysdaleUNKNOWN FlyBase Consortium
Mar 31, 2005·Molecular and Cellular Biology·Haiyan TangWeimin Zhong
Apr 12, 2005·Journal of Molecular Endocrinology·H K KinyamuT K Archer
Feb 16, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Ashok N Hegde, Sudarshan C Upadhya

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Citations

May 26, 2007·Biology of the Cell·Kevin F HaasKendal Broadie
Sep 12, 2014·Apoptosis : an International Journal on Programmed Cell Death·Jessie ColinIsabelle Guénal
Jul 23, 2014·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Michael McMurray
Mar 17, 2007·Molecular and Cellular Neurosciences·Kevin F HaasKendal Broadie

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