A genome-scale metabolic model of the lipid-accumulating yeast Yarrowia lipolytica.

BMC Systems Biology
Nicolas LoiraDavid Sherman

Abstract

Yarrowia lipolytica is an oleaginous yeast which has emerged as an important microorganism for several biotechnological processes, such as the production of organic acids, lipases and proteases. It is also considered a good candidate for single-cell oil production. Although some of its metabolic pathways are well studied, its metabolic engineering is hindered by the lack of a genome-scale model that integrates the current knowledge about its metabolism. Combining in silico tools and expert manual curation, we have produced an accurate genome-scale metabolic model for Y. lipolytica. Using a scaffold derived from a functional metabolic model of the well-studied but phylogenetically distant yeast S. cerevisiae, we mapped conserved reactions, rewrote gene associations, added species-specific reactions and inserted specialized copies of scaffold reactions to account for species-specific expansion of protein families. We used physiological measures obtained under lab conditions to validate our predictions. Y. lipolytica iNL895 represents the first well-annotated metabolic model of an oleaginous yeast, providing a base for future metabolic improvement, and a starting point for the metabolic reconstruction of other species in the Yarro...Continue Reading

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Methods Mentioned

BETA
gene knockouts
gene knock-outs

Software Mentioned

FBA
Flux Balance Analysis ( FBA )
Pathway Tools
The SEED
iIN800
Génolevures
Inparanoid
DB
COBRA Tools
CellDesigner

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