A genome sequence survey of the filarial nematode Brugia malayi: repeats, gene discovery, and comparative genomics

Molecular and Biochemical Parasitology
Claire WhittonMark Blaxter

Abstract

Comparative nematode genomics has thus far been largely constrained to the genus Caenorhabditis, but a huge diversity of other nematode species, and genomes, exist. The Brugia malayi genome is approximately 100 Mb in size, and distributed across five chromosome pairs. Previous genomic investigations have included definition of major repeat classes and sequencing of selected genes. We have generated over 18,000 sequences from the ends of large-insert clones from bacterial artificial chromosome libraries. These end sequences, totalling over 10 Mb of sequence, contain just under 8 Mb of unique sequence. We identified the known Mbo I and Hha I repeat families in the sequence data, and also identified several new repeats based on their abundance. Genomic copies of 17% of B. malayi genes defined by expressed sequence tags have been identified. Nearly one quarter of end sequences can encode peptides with significant similarity to protein sequences in the public databases, and we estimate that we have identified more than 2700 new B. malayi genes. Importantly, 459 end sequences had homologues in other organisms, but lacked a match in the completely sequenced genomes of Caenorhabditis briggsae and Caenorhabditis elegans, emphasising the...Continue Reading

References

Jan 1, 1975·Transactions of the Royal Society of Tropical Medicine and Hygiene·D J McLarenM G Simpson
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1987·Ciba Foundation Symposium·B K SimW F Piessens
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·L A McReynoldsS A Williams
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A M TakacsT W Nilsen
Jan 1, 1987·Annual Review of Genetics·J Hodgkin
Mar 11, 1986·Nucleic Acids Research·R E EllisA R Coulson
Jun 1, 1994·Molecular Phylogenetics and Evolution·J R VanfleterenL Moens
Nov 1, 1995·Molecular and Biochemical Parasitology·M SironiC Genchi
Apr 1, 1996·Molecular and Biochemical Parasitology·M L BlaxterA L Scott
Oct 1, 1996·International Journal for Parasitology·M Blaxter, L Liu
Feb 1, 1997·Molecular and Biochemical Parasitology·N M el-Sayed, J E Donelson
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Sep 2, 1997·Proceedings of the National Academy of Sciences of the United States of America·D EvansT Blumenthal
Mar 24, 1998·Nature·M L BlaxterW K Thomas
May 5, 1998·Molecular and Biochemical Parasitology·I GhoshA L Scott
Aug 12, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·T Blumenthal
Oct 8, 1998·Molecular and Biochemical Parasitology·E M KeddieT R Unnasch
Dec 16, 1998·Science·UNKNOWN C. elegans Sequencing Consortium
Apr 24, 1999·Molecular and Biochemical Parasitology·A P Underwood, A E Bianco
Aug 31, 1999·Parasitology·M BlaxterJ Daub
Dec 10, 1999·Microbial & Comparative Genomics·B E SlatkoM L Blaxter
Mar 25, 2000·International Journal for Parasitology·S A WilliamsM L Blaxter
Jan 11, 2000·Nucleic Acids Research·L SteinJ Spieth
Jan 5, 2001·Nucleic Acids Research·T Sicheritz-Pontén, S G Andersson
Mar 13, 2001·Journal of Bacteriology·L V SunS L O'Neill
Jun 19, 2001·International Journal for Parasitology·R M MaizelsX Zang
Sep 15, 2001·Science·S K KimG S Davidson
Apr 3, 2002·Transactions of the Royal Society of Tropical Medicine and Hygiene·Mark BlaxterUNKNOWN Filarial Genome Project
May 3, 2002·BMC Evolutionary Biology·Jaime E BlairS Blair Hedges
Jun 5, 2002·Genome Research·Avril Coghlan, Kenneth H Wolfe
Oct 5, 2002·Science·Robert A HoltStephen L Hoffman
Oct 26, 2002·BMC Bioinformatics·John ParkinsonMark Blaxter
Nov 5, 2002·Nature Reviews. Genetics·S Blair Hedges
Jan 10, 2003·Nucleic Acids Research·Brigitte BoeckmannMichel Schneider
Jan 16, 2003·Current Biology : CB·A Aziz Aboobaker, Mark L Blaxter

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Citations

Mar 16, 2006·Medical Microbiology and Immunology·M E Viney
Nov 21, 2007·Invertebrate Neuroscience : in·Katherine A SmithJohn Gray
Nov 16, 2004·Nature Genetics·John ParkinsonMark L Blaxter
Nov 24, 2006·PLoS Genetics·David B Guiliano, Mark L Blaxter
May 16, 2006·International Journal for Parasitology·Yong YinMakedonka Mitreva
Feb 14, 2006·International Journal for Parasitology·Peter GeldhofCollette Britton
May 24, 2005·Molecular and Biochemical Parasitology·Fiona J ThompsonMark E Viney
Dec 4, 2012·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Samantha N McNultyPeter U Fischer
Oct 31, 2009·Molecular and Biochemical Parasitology·Catherine B PooleLarry A McReynolds
Apr 13, 2007·Journal of Medical Entomology·Brenda T Beerntsen, Roy J Lowery
Jul 15, 2005·Expert Review of Molecular Diagnostics·Jorge A Huete-PérezConor R Caffrey
Apr 25, 2020·Nature Communications·Jeremy M FosterElodie Ghedin
Aug 16, 2005·Trends in Genetics : TIG·Makedonka MitrevaJames P McCarter
Apr 27, 2021·Parasitology International·Andrea LangelandDamien M O'Halloran

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