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A genome-wide analysis of Cas9 binding specificity using ChIP-seq and targeted sequence capture

bioRxiv

May 22, 2014

Henriette O'GeenDavid Jay Segal

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Abstract

Clustered regularly interspaced short palindromic repeat (CRISPR) RNA-guided nucleases have gathered considerable excitement as a tool for genome engineering. However, questions remain about the specificity of their target site recognition. Most previous studies have examined predicted ...read more

Mentioned in this Paper

Study
DNA Cleavage
Recognition (Psychology)
Chromatin Immunoprecipitation
Genome
RNA
Endonuclease
Genome-Wide Association Study
Site
High-Throughput RNA Sequencing
10
77
Paper Details
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  • A genome-wide analysis of Cas9 binding specificity using ChIP-seq and targeted sequence capture

    bioRxiv

    May 22, 2014

    Henriette O'GeenDavid Jay Segal

    PMID: 990005413

    DOI: 10.1101/005413

    Abstract

    Clustered regularly interspaced short palindromic repeat (CRISPR) RNA-guided nucleases have gathered considerable excitement as a tool for genome engineering. However, questions remain about the specificity of their target site recognition. Most previous studies have examined predicted ...read more

    Mentioned in this Paper

    Study
    DNA Cleavage
    Recognition (Psychology)
    Chromatin Immunoprecipitation
    Genome
    10
    77
    Paper Details
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