A Genome-Wide CRISPR-Cas9 Screen Reveals the Requirement of Host Cell Sulfation for Schmallenberg Virus Infection.

Journal of Virology
Thiprampai ThamamongoodMartin Schwemmle

Abstract

Schmallenberg virus (SBV) is an insect-transmitted orthobunyavirus that can cause abortions and congenital malformations in the offspring of ruminants. Even though the two viral surface glycoproteins Gn and Gc are involved in host cell entry, the specific cellular receptors of SBV are currently unknown. Using genome-wide CRISPR-Cas9 forward screening, we identified 3'-phosphoadenosine 5'-phosphosulfate (PAPS) transporter 1 (PAPST1) as an essential factor for SBV infection. PAPST1 is a sulfotransferase involved in heparan sulfate proteoglycan synthesis encoded by the solute carrier family 35 member B2 gene (SLC35B2). SBV cell surface attachment and entry were largely reduced upon the knockout of SLC35B2, whereas the reconstitution of SLC35B2 in these cells fully restored their susceptibility to SBV infection. Furthermore, treatment of cells with heparinase diminished infection with SBV, confirming that heparan sulfate plays an important role in cell attachment and entry, although to various degrees, heparan sulfate was also found to be important to initiate infection by two other bunyaviruses, La Crosse virus and Rift Valley fever virus. Thus, PAPST1-triggered synthesis of cell surface heparan sulfate is required for the efficie...Continue Reading

References

Mar 1, 1992·The Journal of Cell Biology·M T ShiehP G Spear
Mar 15, 2001·The New England Journal of Medicine·J E McJunkinA Thompson
Apr 30, 2003·The Journal of Biological Chemistry·Shin KamiyamaShoko Nishihara
Jul 18, 2003·The Journal of Biological Chemistry·Heidi BarthThomas F Baumert
Mar 1, 1965·American Journal of Epidemiology·W H THOMPSONR O ANSLOW
Oct 29, 2005·Journal of Virology·Cornelis A M de HaanPeter J M Rottier
Sep 19, 2006·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Onder ErgönülBaşak Dokuzoğuz
Aug 30, 2008·The Journal of General Virology·Matthias HabjanFriedemann Weber
Jul 3, 2009·The Journal of General Virology·Xiaohong ShiRichard M Elliott
Apr 30, 2010·The Journal of General Virology·Ingeborg van KnippenbergRichard M Elliott
Sep 8, 2011·Proceedings of the National Academy of Sciences of the United States of America·Christina L GardnerWilliam B Klimstra
Dec 7, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Yong-Zhen ZhangJianguo Xu
Mar 2, 2012·Emerging Infectious Diseases·Bernd HoffmannMartin Beer
Apr 21, 2012·Veterinary Microbiology·S BilkB Hoffmann
Apr 26, 2012·Molecular Systems Biology·Berend SnijderLucas Pelkmans
May 9, 2012·Handbook of Experimental Pharmacology·Pernilla Carlsson, Lena Kjellén
Jun 8, 2012·Proceedings of the National Academy of Sciences of the United States of America·David M RosmarinHidde L Ploegh
Sep 28, 2012·Journal of Virology·S M de BoerB J Bosch
Oct 3, 2012·Transboundary and Emerging Diseases·N De ReggeA B Cay
Oct 11, 2012·Epidemiology and Infection·M BeerW H M van der Poel
May 23, 2013·PLoS Pathogens·Thomas A BowdenJuha T Huiskonen
Dec 18, 2013·Science·Ophir ShalemFeng Zhang
Apr 29, 2014·Preventive Veterinary Medicine·Kerstin WernikeMartin Beer
Sep 10, 2014·Nature Reviews. Microbiology·Richard M Elliott
Dec 3, 2014·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Detlev H KrugerBoris Klempa
Jan 19, 2016·Nature Biotechnology·John G DoenchDavid E Root
May 18, 2018·Nature·Rong ZhangMichael S Diamond
Aug 7, 2018·PLoS Pathogens·Lindsey R Robinson-McCarthySean P J Whelan
Feb 23, 2019·Nature Communications·Jan HellertFélix A Rey
Apr 26, 2019·Proceedings of the National Academy of Sciences of the United States of America·Yun LiRudolf Jaenisch
Dec 1, 2019·Molecular Therapy : the Journal of the American Society of Gene Therapy·Amanda M DudekLuk H Vandenberghe

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.