Nov 12, 2013

Electrostatic and bending energies predict staggering and splaying in nonmuscle myosin II minifilaments

BioRxiv : the Preprint Server for Biology
Biranchi PatraAnimesh Ray

Abstract

Recent experiments with super-resolution live cell microscopy revealed that nonmuscle myosin II minifilaments are much more dynamic than formerly appreciated, often showing plastic processes such as splitting, concatenation and stacking. Here we combine sequence information, electrostatics and elasticity theory to demonstrate that the parallel staggers at 14.3, 43.2 and 72 nm have a strong tendency to splay their heads away from the minifilament, thus potentially initiating the diverse processes seen in live cells. In contrast, the straight antiparallel stagger with an overlap of 43 nm is very stable and likely initiates minifilament nucleation. Using stochastic dynamics in a newly defined energy landscape, we predict that the optimal parallel staggers between the myosin rods are obtained by a trial-and-error process in which two rods attach and re-attach at different staggers by rolling and zipping motion. The experimentally observed staggers emerge as the configurations with the largest contact times. We find that contact times increase from isoforms C to B to A, that A-B-heterodimers are surprisingly stable and that myosin 18A should incorporate into mixed filaments with a small stagger. Our findings suggest that nonmuscle m...Continue Reading

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Mentioned in this Paper

Genome-Wide Association Study
Biochemical Pathway
Abnormal Degeneration
RNA Chemical Synthesis
Genome
Genes
DNA-Directed RNA Polymerase
RNA Polymerase Assembly Pathway
Complex (molecular entity)
Nerve Degeneration

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