A genome-wide transcription analysis of a fungal riboflavin overproducer

Journal of Biotechnology
Marvin KarosJosé Luis Revuelta

Abstract

The production of many fine chemicals such as vitamins and amino acids is carried out in bioreactors using microorganisms. Usually, these strains are developed from wild-type organisms by classical mutation and selection. After several generations of strain improvement, no further enhancement can be achieved. Therefore, metabolic engineering (ME) is a rational approach to optimise such producer organisms beyond this point, or for starting all over from the beginning. Metabolic Engineering involves detailed analysis of the organism's metabolic and genetic properties, leading to the identification of new target genes. The fungal riboflavin overproducer Ashbya gossypii converts vegetable oil to vitamin B2 in a "one-step reaction". The productivity and selectivity of this microorganism have been optimised significantly over the years, first following a classical approach and now a rational one. The improvement is based on our understanding of vitamin B2 metabolism. We have been able to selectively enhance the pathways that are necessary for the formation of riboflavin and to inhibit those leading to unwanted side products. New targets for further improvements of this process have been found using a genome-wide transcript expression...Continue Reading

References

Feb 15, 1979·European Journal of Biochemistry·F HilgerV Stalon
Apr 2, 1992·Nature·D Ursic, M R Culbertson
Jan 1, 1992·Journal of Chemical Technology and Biotechnology·E J Vandamme
Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·B Daignan-Fornier, G R Fink
Jul 1, 1967·Journal of Chromatography·P CerlettiM A Giovenco
Oct 1, 1994·Molecular Biology of the Cell·D UrsicM R Culbertson
Mar 1, 1993·Nature Genetics·W Gish, D J States
Sep 7, 2001·Genes & Development·D D'Amours, S P Jackson
Apr 6, 2002·Applied Microbiology and Biotechnology·J H MartensD Jahn

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Citations

Sep 15, 2005·Applied Microbiology and Biotechnology·Tatsuya Kato, Enoch Y Park
Apr 28, 2010·Applied Microbiology and Biotechnology·Orquídea RibeiroMerja Penttilä
May 17, 2011·Applied Microbiology and Biotechnology·Enoch Y ParkTatsuya Kato
Dec 22, 2011·Biotechnology Letters·Tatsuya Kato, Enoch Y Park
Jun 8, 2011·Microbiology and Molecular Biology Reviews : MMBR·Charles A Abbas, Andriy A Sibirny
Jul 31, 2008·Microbial Cell Factories·Hans MarxMichael Sauer
Oct 16, 2015·Microbial Cell Factories·Rodrigo Ledesma-AmaroJosé Luis Revuelta
Feb 14, 2006·FEMS Microbiology Reviews·Jose L Adrio, Arnold L Demain
Feb 18, 2011·Bioengineered Bugs·Jose-Luis Adrio, Arnold L Demain
Oct 4, 2016·Journal of Industrial Microbiology & Biotechnology·José Luis RevueltaAlberto Jiménez
Jul 1, 2008·Microbial Biotechnology·Sergio Sanchez, Arnold L Demain

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