A genomic duplication is associated with ectopic eomesodermin expression in the embryonic chicken comb and two duplex-comb phenotypes

PLoS Genetics
Ben DorshorstLeif Andersson

Abstract

Duplex-comb (D) is one of three major loci affecting comb morphology in the domestic chicken. Here we show that the two Duplex-comb alleles, V-shaped (D*V) and Buttercup (D*C), are both associated with a 20 Kb tandem duplication containing several conserved putative regulatory elements located 200 Kb upstream of the eomesodermin gene (EOMES). EOMES is a T-box transcription factor that is involved in mesoderm specification during gastrulation. In D*V and D*C chicken embryos we find that EOMES is ectopically expressed in the ectoderm of the comb-developing region as compared to wild-type embryos. The confinement of the ectopic expression of EOMES to the ectoderm is in stark contrast to the causal mechanisms underlying the two other major comb loci in the chicken (Rose-comb and Pea-comb) in which the transcription factors MNR2 and SOX5 are ectopically expressed strictly in the mesenchyme. Interestingly, the causal mutations of all three major comb loci in the chicken are now known to be composed of large-scale structural genomic variants that each result in ectopic expression of transcription factors. The Duplex-comb locus also illustrates the evolution of alleles in domestic animals, which means that alleles evolve by the accumul...Continue Reading

References

Mar 1, 1991·The Journal of Heredity·R G Somes
Mar 1, 1991·The Journal of Heredity·R G Somes
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Dec 31, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Chris ShowellFrank L Conlon
Jan 23, 2004·Genetics, Selection, Evolution : GSE·F PitelM Tixier-Boichard
Dec 14, 2004·Nature·Gane Ka-Shu WongUNKNOWN International Chicken Polymorphism Map Consortium
Jan 8, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Sarah A Maas, John F Fallon
Jun 21, 2005·Genome Research·Gregory M CooperArend Sidow
Jun 28, 2005·Developmental Biology·Ralph S MarcucioJill A Helms
May 15, 1908·Science·R C Punnett, W Bateson
May 20, 2009·Bioinformatics·Heng Li, Richard Durbin
Jun 13, 2009·PLoS Genetics·Dominic WrightLeif Andersson
Jul 15, 2009·Nature Reviews. Genetics·P J HastingsGrzegorz Ira
Dec 17, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Bárbara PernauteMiguel Manzanares
Jan 8, 2011·Pigment Cell & Melanoma Research·Ulrika GunnarssonLeif Andersson
Feb 18, 2011·Pediatric Annals·Robert W Hered
Apr 6, 2011·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Ian C DunnMegan G Davey
Jun 2, 2011·BMC Genomics·Martien A M GroenenHans H Cheng
Jun 28, 2012·PloS One·Ivica MedugoracStefan Krebs
Nov 16, 2012·Proceedings of the National Academy of Sciences of the United States of America·Carl-Johan RubinLeif Andersson
Apr 23, 2013·Current Opinion in Genetics & Development·Leif Andersson
May 30, 2013·PloS One·Aurélie Allais-BonnetAurélien Capitan
Jul 6, 2014·Genetics, Selection, Evolution : GSE·Sophie RothammerIvica Medugorac

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Citations

Oct 20, 2015·Upsala Journal of Medical Sciences·Leif Andersson
Oct 27, 2017·Genetics, Selection, Evolution : GSE·Rabia LetaiefMekki Boussaha
Oct 30, 2015·Bioinformatics and Biology Insights·Dominic Wright
Mar 20, 2015·PLoS Genetics·Denis Headon
Aug 23, 2019·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Daniel Núñez-LeónMarcelo R Sánchez-Villagra
Aug 8, 2015·G3 : Genes - Genomes - Genetics·Yanfa SunJie Wen
May 29, 2016·G3 : Genes - Genomes - Genetics·Małgorzata PilotWiesław Bogdanowicz
Feb 13, 2019·Trends in Plant Science·Zoe N Lye, Michael D Purugganan
Jun 2, 2021·Animal Genetics·R A Lawal, O Hanotte

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Methods Mentioned

BETA
chip
PCR
reverse transcription-PCR
genotyping

Software Mentioned

AxioVision
geNorm
EOMES
BLAT
Copy Caller
Burrows Wheeler Aligner ( BWA )
Genome Analysis Toolkit ( GATK ) Unified Genotyper
Picard Tools
UCSC genome browser
Genomic Evolutionary Rate Profiling ( GERP )

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