A genomic screen revealing the importance of vesicular trafficking pathways in genome maintenance and protection against genotoxic stress in diploid Saccharomyces cerevisiae cells

PloS One
Kamil KrolAdrianna Skoneczna

Abstract

The ability to survive stressful conditions is important for every living cell. Certain stresses not only affect the current well-being of cells but may also have far-reaching consequences. Uncurbed oxidative stress can cause DNA damage and decrease cell survival and/or increase mutation rates, and certain substances that generate oxidative damage in the cell mainly act on DNA. Radiomimetic zeocin causes oxidative damage in DNA, predominantly by inducing single- or double-strand breaks. Such lesions can lead to chromosomal rearrangements, especially in diploid cells, in which the two sets of chromosomes facilitate excessive and deleterious recombination. In a global screen for zeocin-oversensitive mutants, we selected 133 genes whose deletion reduces the survival of zeocin-treated diploid Saccharomyces cerevisiae cells. The screen revealed numerous genes associated with stress responses, DNA repair genes, cell cycle progression genes, and chromatin remodeling genes. Notably, the screen also demonstrated the involvement of the vesicular trafficking system in cellular protection against DNA damage. The analyses indicated the importance of vesicular system integrity in various pathways of cellular protection from zeocin-dependent ...Continue Reading

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Citations

Nov 15, 2015·Cell Cycle·Anna DeregowskaMaciej Wnuk
Jan 25, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Petronela ImreovaIvan Chalupa
May 2, 2019·Oncotarget·Sophia B ChernikovaJ Martin Brown
Nov 5, 2020·International Journal of Molecular Sciences·Daria S SpasskayaDmitry S Karpov

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Methods Mentioned

BETA
chemical treatment
PCR
ubiquitination
flow cytometry
fluorescence-activated cell sorting
FACS
electrophoresis
gene knock-out
feature extraction

Software Mentioned

Acuity
GenePix Pro
Cytoscape
Excel
BINGO

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