A GeNorm algorithm-based selection of reference genes for quantitative real-time PCR in skin biopsies of healthy dogs and dogs with atopic dermatitis

Veterinary Immunology and Immunopathology
Yvette M SchlotterLouis C Penning

Abstract

Quantitative real time PCR (Q-PCR) is the method of choice to study mRNA expression levels. Since Q-PCR is very sensitive, normalization of the data with stably expressed reference genes if of utmost importance. The stability of reference genes depends on the tissue and the species of interest. Therefore, evaluation of the stability of reference genes must be performed for each new tissue and species under study. The stability of B2M, GAPDH, HPRT, SRPR, hnRNPH, GUSB, RPL8, RPS5, and RPS19 was analyzed with the GeNorm software in snap frozen canine skin biopsies. Healthy dogs (n=7) and dogs with confirmed atopic dermatitis (n=28) were included. Lesional and non-lesional skin was analyzed. The study indicated that the most appropriate reference genes in canine skin are the ribosomal gene products RPL8, RPS5 and RPS19 besides GUSB and HPRT. As little as three reference genes will reveal highly reliable Q-PCR calculations.

References

May 25, 2004·Science·Heidi G ParkerLeonid Kruglyak
Oct 8, 2005·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Falk OhlKlaus Jung
Dec 13, 2005·Nature·Kerstin Lindblad-TohEric S Lander
Jul 18, 2006·Analytical Biochemistry·Bas BrinkhofLouis C Penning
Apr 5, 2007·The Journal of Heredity·David R SarganElaine A Ostrander
Oct 2, 2007·Veterinary Immunology and Immunopathology·Louis C PenningHerman A W Hazewinkel

❮ Previous
Next ❯

Citations

Feb 10, 2011·BMC Research Notes·Christine J PiekLouis C Penning
Jul 13, 2012·Research in Veterinary Science·Sirin TheerawatanasirikulGunnaporn Suriyaphol
Jun 21, 2011·Veterinary Immunology and Immunopathology·Yvette M SchlotterTon Willemse
Jun 17, 2015·Bioscience, Biotechnology, and Biochemistry·Zubi LiuHai Liao
Mar 19, 2014·Journal of Animal Physiology and Animal Nutrition·J SchumannH Fuhrmann
Nov 13, 2010·Microbes and Infection·Yan LiuXue-jie Yu
Feb 5, 2013·Current Eye Research·Aneliya M HaritovaJohanna Fink-Gremmels
Apr 26, 2016·Journal of Veterinary Internal Medicine·K SandersS Galac
Oct 19, 2016·The Journal of Pathology·Silvia SookoianCarlos J Pirola
Nov 16, 2011·Journal of Bioinformatics and Computational Biology·Ameya DeoAngelica Lindlöf
Dec 17, 2011·International Journal of Molecular Sciences·Simone PelettoPier Luigi Acutis
Jul 14, 2019·Veterinary and Comparative Oncology·Karin SandersSara Galac
May 1, 2010·The Journal of Veterinary Medical Science·Hirotaka TomiyasuHajime Tsujimoto
Jun 11, 2019·Cellular and Molecular Gastroenterology and Hepatology·Luca Di MartinoFabio Cominelli

❮ Previous
Next ❯

Related Concepts

Related Feeds

Atopic Dermatitis

Atopic dermatitis is a chronic inflammatory genetically determined disease of the skin marked by increased ability to form reagin (IgE), with increased susceptibility to allergic rhinitis and asthma, and hereditary disposition to a lowered threshold for pruritus. Discover the latest research on atopic dermatitis here.