A GFP-transfected HFWT cell line, GHINK-1, as a novel target for non-RI activated natural killer cytotoxicity assay

Human Cell
Hideki HaradaTadao Ohno

Abstract

An anchorage-dependent Wilms' tumor cell line, HFWT, has been found to be extremely susceptible to human natural killer (NK) cells. Here we established a transfectant of HFWT with the green fluorescence protein (GFP) gene, designated GHINK-1 cells, to apply to the activated NK cytotoxicity assay without radioisotope labeling. After being co-cultured with CD3 CD56+ NK cells, GHINK-1 cells released GFP into the medium. The intensity of the fluorescence from the released GFP correlated almost exactly with the radioactivity of a standard 51Cr-release assay performed with suspension-cultured K562 cells. Because it does not require separation of the remaining live target cells by centrifugation, the non-radioisotopic GFP release assay with GHINK-1 cells is a convenient alternative for monitoring human activated NK killing activity.

References

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Apr 3, 2002·Japanese Journal of Cancer Research : Gann·Hideki HaradaTadao Ohno
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Citations

Sep 14, 2006·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Séverine AllegraMarie-Paule Vasson
Nov 24, 2005·European Journal of Immunology·Hideki HaradaSeiji Okada

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