A glandular trichome-specific monoterpene alcohol dehydrogenase from Artemisia annua

Phytochemistry
Devin R PolichukPatrick S Covello

Abstract

The major components of the isoprenoid-rich essential oil of Artemisia annua L. accumulate in the subcuticular sac of glandular secretory trichomes. As part of an effort to understand isoprenoid biosynthesis in A. annua, an expressed sequence tag (EST) collection was investigated for evidence of genes encoding trichome-specific enzymes. This analysis established that a gene denoted Adh2, encodes an alcohol dehydrogenase and shows a high expression level in glandular trichomes relative to other tissues. The gene product, ADH2, has up to 61% amino acid identity to members of the short chain alcohol dehydrogenase/reductase (SDR) superfamily, including Forsythia x intermedia secoisolariciresinol dehydrogenase (49.8% identity). Through in vitro biochemical analysis, ADH2 was found to show a strong preference for monoterpenoid secondary alcohols including carveol, borneol and artemisia alcohol. These results indicate a role for ADH2 in monoterpenoid ketone biosynthesis in A. annua glandular trichomes.

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Citations

Nov 22, 2011·Insect Biochemistry and Molecular Biology·Rubi Figueroa-TeranClaus Tittiger
Mar 17, 2016·Journal of Asian Natural Products Research·Mohammed TalbiTarik Ainane
Jun 15, 2011·The FEBS Journal·Sho OkamotoRyutaro Utsumi
Sep 18, 2012·Plant Biotechnology Journal·B Markus Lange, Glenn W Turner
May 24, 2013·International Journal of Molecular Sciences·Damian Paul DrewHenrik Toft Simonsen
Jan 10, 2019·Journal of Experimental Botany·Bernd Markus Lange, Narayanan Srividya
Apr 14, 2021·Applied Microbiology and Biotechnology·Michael HoferVolker Sieber
Jul 16, 2021·ChemCatChem·Andrea M ChániqueRobert Kourist

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