A global lipid map defines a network essential for Zika virus replication.

Nature Communications
Hans C LeierFikadu G Tafesse

Abstract

Zika virus (ZIKV), an arbovirus of global concern, remodels intracellular membranes to form replication sites. How ZIKV dysregulates lipid networks to allow this, and consequences for disease, is poorly understood. Here, we perform comprehensive lipidomics to create a lipid network map during ZIKV infection. We find that ZIKV significantly alters host lipid composition, with the most striking changes seen within subclasses of sphingolipids. Ectopic expression of ZIKV NS4B protein results in similar changes, demonstrating a role for NS4B in modulating sphingolipid pathways. Disruption of sphingolipid biosynthesis in various cell types, including human neural progenitor cells, blocks ZIKV infection. Additionally, the sphingolipid ceramide redistributes to ZIKV replication sites, and increasing ceramide levels by multiple pathways sensitizes cells to ZIKV infection. Thus, we identify a sphingolipid metabolic network with a critical role in ZIKV replication and show that ceramide flux is a key mediator of ZIKV infection.

References

Mar 10, 2001·Nature Genetics·K BejaouiR H Brown
Dec 1, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·H BirbesL M Obeid
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Aug 3, 2002·The Journal of Biological Chemistry·Chiara LubertoGary K Smith
Jul 7, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ralf Bartenschlager, Thomas Pietschmann
Aug 15, 2006·The Journal of Biological Chemistry·Fikadu Geta TafesseJoost C M Holthuis
Oct 20, 2006·Biochimica Et Biophysica Acta·Antonio DelgadoGemma Fabrias
Nov 14, 2006·Nucleic Acids Research·Manish SudShankar Subramaniam
Apr 24, 2007·The Journal of Biological Chemistry·Fikadu Geta TafesseJoost C M Holthuis
Dec 11, 2007·Nature Biotechnology·Seng-Lai TanMichael G Katze
May 6, 2008·Bioinformatics·Ashoka D PolpitiyaRichard D Smith
Jul 1, 2008·Nature Reviews. Microbiology·Catherine L MurrayCharles M Rice
Jul 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·D-S LeeA-L Barabási
Apr 3, 2009·Nature·Rajat SinghMark J Czaja
Jun 10, 2009·The Journal of Cell Biology·Ana M VacaruJoost C M Holthuis
Oct 27, 2009·Trends in Endocrinology and Metabolism : TEM·Gulam H SyedAleem Siddiqui
Dec 8, 2009·Science·Jan E CaretteThijn R Brummelkamp
Jan 2, 2010·Science·Daniel Lingwood, Kai Simons
Feb 26, 2010·Nature·David K BreslowJonathan S Weissman
Apr 22, 2010·Cellular Signalling·Judith MesicekRichard Kolesnick
Oct 6, 2010·Advances in Experimental Medicine and Biology·Christopher R GaultYusuf A Hannun
Oct 6, 2010·Advances in Experimental Medicine and Biology·Johnny StibanAnthony H Futerman
Nov 16, 2010·Cell Host & Microbe·Nicholas S Heaton, Glenn Randall
Dec 15, 2010·Bioinformatics·Michael E SmootTrey Ideker
Dec 18, 2010·Nature Reviews. Genetics·Albert-László BarabásiJoseph Loscalzo
Mar 29, 2011·Chemistry & Biology·Richard W Gross, Xianlin Han
Apr 23, 2011·The Journal of Biological Chemistry·Jeffrey E McLeanZahra Zakeri
Jun 23, 2011·The Journal of Biological Chemistry·Yusuf A Hannun, Lina M Obeid
Aug 20, 2011·Bioinformatics·Melissa M MatzkeBobbie-Jo M Webb-Robertson
Aug 26, 2011·Nature·Jan E CaretteThijn R Brummelkamp
Nov 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·Françoise M RoelantsJeremy Thorner
Mar 30, 2012·PLoS Pathogens·Rushika PereraRichard J Kuhn
Apr 12, 2012·Bioinformatics·Maureen A SartorAlla Karnovsky
Nov 16, 2012·Analytical Chemistry·Alysha M De LiveraTerence P Speed
Jan 15, 2013·Journal of Lipid Research·Torahiko TanakaMakoto Makishima
Apr 12, 2013·Proceedings of the National Academy of Sciences of the United States of America·Fikadu G TafesseHidde L Ploegh
Apr 13, 2013·Handbook of Experimental Pharmacology·Krishna P Bhabak, Christoph Arenz
Dec 18, 2013·Science·Ophir ShalemFeng Zhang

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Citations

Oct 30, 2020·ELife·Anya J O'NealJoao Hf Pedra
Nov 5, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Florian GeltingerMark Rinnerthaler
Jan 31, 2021·The Journal of Biological Chemistry·Eric BarklisFikadu G Tafesse
Mar 20, 2021·Frontiers in Physiology·Octavio A C TalyuliGabriela O Paiva-Silva
Apr 14, 2021·Cellular and Molecular Life Sciences : CMLS·Yali Ci, Lei Shi
May 26, 2021·Expert Review of Proteomics·Jennifer E Kyle
Jun 3, 2021·International Journal of Molecular Sciences·Nadine Beckmann, Katrin Anne Becker
Jun 28, 2021·Current Opinion in Chemical Biology·Alec Millner, G Ekin Atilla-Gokcumen
Jul 13, 2021·Frontiers in Cellular and Infection Microbiology·Jerica Isabel L ReyesKozo Watanabe
Jul 3, 2021·Biomedicines·Philma Glora MuthurajSathish Kumar Natarajan

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Methods Mentioned

BETA
transfection
PCA
gene-trap
SMase
superresolution microscopy
light microscopy
biosensors
glycosylation
transfections
scraping

Software Mentioned

FlowJo
Imaris
MetDisease Cytoscape app
Prism
MetScape
LIQUID
MATLAB
GraphPad
Metab2MeSH
Cytoscape

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