PMID: 6412678Jul 1, 1983Paper

A glucuronyltransferase involved in glucuronoxylan synthesis in pea (Pisum sativum) epicotyls

The Biochemical Journal
K W Waldron, Christopher T Brett

Abstract

A particulate enzyme preparation made from epicotyls of 1-week-old etiolated pea (Pisum sativum) seedlings was shown to incorporate glucuronic acid from UDP-D-[U-14C]glucuronic acid into a hemicellulosic polysaccharide. Optimum conditions for the incorporation include the presence of Mn2+ ions at between 4 and 10 mmol/litre and a pH between 5 and 6. UDP-D-xylose at 1 mmol/litre allows incorporation to continue for at least 8 h. In its absence, the reaction stops within 30 min. Analysis of the product by partial and total acid hydrolysis, followed by paper chromatography or electrophoresis, indicates that the polysaccharide produced is a glucuronoxylan.

Citations

Jan 1, 1990·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·C W WhiteE S Jacobson
Jun 9, 2005·Journal of Plant Physiology·Yao-Ting Wu, Jin-Yuan Liu
Feb 26, 2014·Critical Reviews in Biochemistry and Molecular Biology·Zhangying Hao, Debra Mohnen
Apr 26, 2020·International Journal of Molecular Sciences·Ilaria MarcotuliAgata Gadaleta
Sep 13, 2002·Plant Physiology·Andrea Celia PorchiaHenrik Vibe Scheller
Apr 9, 2010·Plant Physiology·Ahmed Faik

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