A glue-based, screw-free method for implantation of intra-cranial electrodes in young mice

Journal of Neuroscience Methods
Chiping WuLiang Zhang

Abstract

Intra-cranial electroencephalographic recordings are increasingly employed in mice because of the availability of genetically manipulated mouse models. Currently, dental acrylic and anchoring screws are used to cement implanted electrodes. This technique works well for adult animals but often encounters difficulty when employed in young mice because their skulls are not strong enough to bear the anchoring screws. Here we describe a novel method favorable for implantation of intra-cranial electrodes in mice as young as postnatal 18 days and suitable for long-term intra-cranial electroencephalographic recordings. Our approach is to construct a multi-electrode assembly according to the desired stereotaxic coordinates of intra-cranial recordings and to secure the implanted electrode assembly to the skull via glue rather than dental acrylic/anchoring screws. The surgical operation for such electrode implantation is relatively quick and rarely associated with complications such as infection, bleeding, neurological deficits, spontaneous seizures or behavioral disturbances. The implanted electrodes are stable, allowing repeated monitoring for several months. Data obtained by simultaneous intra-hippocampal and intra-cortical recordings ...Continue Reading

Citations

May 9, 2012·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Jaehyung KimJin Woo Chang
Sep 17, 2015·Journal of Neuroscience Methods·Katie LidsterMark J Prescott
Jan 5, 2010·Neurobiology of Disease·Jennifer Anne D'CruzJames H Eubanks
Dec 30, 2010·IEEE Transactions on Bio-medical Engineering·Dong-Hyun BaekSang-Hoon Lee
Oct 27, 2015·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Liang ZhangJames H Eubanks
May 7, 2015·Experimental Neurology·Chiping WuLiang Zhang
Mar 21, 2013·Experimental Neurology·Youssef Hanna El-HayekLiang Zhang
Oct 6, 2009·Current Protocols in Neuroscience·C Martín del CampoMiguel A Cortez Freire
Feb 13, 2015·Journal of Korean Neurosurgical Society·Jinhyung KimJin Woo Chang
Sep 30, 2017·Frontiers in Behavioral Neuroscience·Na-Ryum BinLiang Zhang
Oct 16, 2020·International Journal of Molecular Sciences·Mark S AquilinoPeter L Carlen

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