A glutathione S-transferases isozyme (bGST 5.8) involved in the metabolism of 4-hydroxy-2-trans-nonenal is localized in bovine lens epithelium

Experimental Eye Research
S K SrivastavaY C Awasthi

Abstract

Previous studies have suggested that a group of GST isozymes (bGST 5.8) with substrate preference for 4-hydroxy-2-trans-nonenal (4-HNE) were present in bovine retina, cornea, iris-ciliary body and sclera, but not in lens. The present studies demonstrate that bGST 5.8 is present in bovine lens epithelium and absent in the cortex and nucleus. Immunochemical studies demonstrated that the enzyme is selectively expressed in epithelium where it can be induced by about 2.5-fold when the lenses are cultured in Medium-199 for 24 hr in the presence of 10 microM BHT. bGST 5.8 was purified to homogeneity from the epithelium using immunoaffinity chromatography. Upon SDS-PAGE, the enzyme showed a single band corresponding to an M(r) value of 25 kDa and its CNBr-peptide maps in SDS-gels were identical to those of the isozymes of this group of GSTs reported previously. The enzyme exhibited high activity towards 4-HNE, and showed glutathione peroxidase activity towards phospholipid hydroperoxides. The Km values of the enzyme for 4-HNE (57 microM from control and 52 microM from BHT-treated) were in the same range as those reported for GSTs 5.8 of human ocular tissues. However, the Kcat value of the lens epithelium enzyme for 4-HNE (15.4 mol mol-...Continue Reading

Citations

Jul 29, 2004·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·James C VeltmanHenry F Edelhauser
Aug 26, 1999·The British Journal of Ophthalmology·N OhguroY Tano
May 28, 2013·Oxidative Medicine and Cellular Longevity·Umesh C S Yadav, Kota V Ramana
Apr 26, 2017·Drug Metabolism Reviews·Upendra A ArgikarAndrea J Bushee
Feb 15, 2019·Expert Opinion on Therapeutic Patents·Luca Quattrini, Concettina La Motta
Sep 7, 2000·Experimental Eye Research·J Jimenez-Asensio, D Garland
Mar 14, 1998·Toxicology and Applied Pharmacology·R J MorrisonS Awasthi

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