A glycine insertion in the estrogen-related receptor (ERR) is associated with enhanced expression of three cytochrome P450 genes in transgenic Drosophila melanogaster

PloS One
Weilin SunBarry R Pittendrigh

Abstract

Insecticide-resistant Drosophila melanogaster strains represent a resource for the discovery of the underlying molecular mechanisms of cytochrome P450 constitutive over-expression, even if some of these P450s are not directly involved in the resistance phenotype. For example, in select 4,4'-dichlorodiphenyltrichloroethane (DDT) resistant strains the glucocorticoid receptor-like (GR-like) potential transcription factor binding motifs (TFBMs) have previously been shown to be associated with constitutively differentially-expressed cytochrome P450s, Cyp12d1, Cyp6g2 and Cyp9c1. However, insects are not known to have glucocorticoids. The only ortholog to the mammalian glucocorticoid receptor (GR) in D. melanogaster is an estrogen-related receptor (ERR) gene, which has two predicted alternative splice isoforms (ERRa and ERRb). Sequencing of ERRa and ERRb in select DDT susceptible and resistant D. melanogaster strains has revealed a glycine (G) codon insertion which was only observed in the ligand binding domain of ERR from the resistant strains tested (ERR-G). Transgenic flies, expressing the ERRa-G allele, constitutively over-expressed Cyp12d1, Cyp6g2 and Cyp9c1. Only Cyp12d1 and Cyp6g2 were over-expressed in the ERRb-G transgenic fl...Continue Reading

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Mar 18, 2016·Annual Review of Entomology·Thomas Van Leeuwen, Wannes Dermauw
Dec 1, 2015·PLoS Genetics·Michael A NajarroStuart J Macdonald
Nov 28, 2018·Archives of Insect Biochemistry and Physiology·GuiLu Zhang, WenJun Zhang
Jun 17, 2020·BMC Microbiology·Gloria BartoloMikhail Martchenko Shilman
Dec 5, 2020·Scientific Reports·Guillaume BordetAlexei V Tulin

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BETA
transgenic
PCR

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