A glycophospholipid membrane anchor acts as an apical targeting signal in polarized epithelial cells.

The Journal of Cell Biology
M P LisantiE Rodriguez-Boulan

Abstract

Glycosyl-phosphatidylinositol- (GPI) anchored proteins contain a large extracellular protein domain that is linked to the membrane via a glycosylated form of phosphatidylinositol. We recently reported the polarized apical distribution of all endogenous GPI-anchored proteins in the MDCK cell line (Lisanti, M. P., M. Sargiacomo, L. Graeve, A. R. Saltiel, and E. Rodriguez-Boulan. 1988. Proc. Natl. Acad. Sci. USA. 85:9557-9561). To study the role of this mechanism of membrane anchoring in targeting to the apical cell surface, we use here decay-accelerating factor (DAF) as a model GPI-anchored protein. Endogenous DAF was localized on the apical surface of two human intestinal cell lines (Caco-2 and SK-CO15). Recombinant DAF, expressed in MDCK cells, also assumed a polarized apical distribution. Transfer of the 37-amino acid DAF signal for GPI attachment to the ectodomain of herpes simplex glycoprotein D (a basolateral antigen) and to human growth hormone (a regulated secretory protein) by recombinant DNA methods resulted in delivery of the fusion proteins to the apical surface of transfected MDCK cells. These results are consistent with the notion that the GPI anchoring mechanism may convey apical targeting information.

References

Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·E Rodriguez Boulan, D D Sabatini
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Apr 1, 1989·The Journal of Cell Biology·I W CarasS R Williams
Jan 1, 1989·Annual Review of Physiology·E Rodriguez-Boulan, P J Salas
Aug 18, 1989·Science·E Rodriguez-Boulan, W J Nelson
Mar 1, 1989·The Journal of Membrane Biology·M SargiacomoE Rodriguez-Boulan
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M P LisantiE Rodriguez-Boulan
Jan 1, 1985·Annual Review of Biophysics and Biophysical Chemistry·T E Thompson, T W Tillack
Jan 1, 1988·Annual Review of Biochemistry·M A Ferguson, A F Williams
Jul 1, 1988·Trends in Biochemical Sciences·G van Meer
Jan 1, 1988·Journal of Cellular Biochemistry·G van Meer, K Simons
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·G L WaneckR A Flavell
Oct 1, 1987·The Journal of Cell Biology·G van MeerK Simons
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J D BangsP T Englund
Sep 13, 1985·Science·R E Pagano, R G Sleight
Apr 1, 1973·Virology·F L Graham, A J van der Eb
Apr 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·H D NiallF C Greenwood
Nov 11, 1983·Nucleic Acids Research·C M GormanR Reeves

❮ Previous
Next ❯

Citations

May 8, 2000·The Prostate·F G KravetsS A Khan
Feb 1, 1997·Journal of Neurobiology·S K PowellM E Hatten
Aug 18, 1999·Electrophoresis·D J SherrierP Dupree
Aug 31, 2001·Journal of Cellular Biochemistry·D R PremkumarM E Medof
Jun 1, 1993·Microscopy Research and Technique·S R Glasser, J Mulholland
Oct 1, 1993·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·W J Nelson
Jul 1, 1990·The Journal of Membrane Biology·M P LisantiA R Saltiel
Jan 1, 1993·Molecular Neurobiology·C Faivre-Sarrailh, G Rougon
Jan 1, 1993·Immunologic Research·I A RooneyJ P Atkinson
Oct 13, 2011·The Journal of Membrane Biology·Randi G BjaeldeHelle A Praetorius
Jun 21, 1991·Biochemical Pharmacology·A R SaltielA A Aderem
May 1, 1992·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·S TakesueY Takesue
Aug 1, 1992·Current Opinion in Cell Biology·K S Matlin
Aug 1, 1994·Current Opinion in Cell Biology·K Matter, I Mellman
Nov 1, 1992·Trends in Cell Biology·D A Brown
Nov 1, 1996·Critical Reviews in Oncology/hematology·H Nakakuma, T Kawaguchi
Jun 12, 2003·Experimental Cell Research·Elizabeth S StuartLeonard C Norkin
Feb 5, 2003·Progress in Biophysics and Molecular Biology·M CereijidoM R García-Villegas
Dec 22, 1999·Pharmacology & Therapeutics·C Saunders, L E Limbird
Aug 1, 1997·Current Opinion in Cell Biology·M G Roth, P C Sternweis
Nov 24, 2001·Trends in Cell Biology·W J Nelson, C Yeaman
Jul 17, 1999·Trends in Cell Biology·E Rodriguez-Boulan, A Gonzalez
Apr 4, 2002·Traffic·Kyujeong AhnAnn H Erickson
Apr 25, 2012·Cell Research·Xinwang CaoKai Simons
Mar 2, 2005·Nature Reviews. Molecular Cell Biology·Enrique Rodriguez-BoulanAnne Müsch
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M P LisantiE Rodriguez-Boulan
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D BrownW S Sly
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S FukuokaG A Scheele
Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M S GhandourW S Sly
Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S FukuokaG A Scheele
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J MoroianuB L Vallee
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·D P JasmerT C McGuire

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.