A glycoprotein I- and glycoprotein E-deficient mutant of infectious laryngotracheitis virus exhibits impaired cell-to-cell spread in cultured cells

Archives of Virology
Joanne M DevlinJ R Gilkerson

Abstract

In alphaherpesviruses, glycoprotein I (gI) and glycoprotein E (gE) form a heterodimer that functions in cell-to-cell spread of the virus. Generally, alphaherpesvirus mutants that lack these glycoproteins are replication competent in cell culture but show a reduced capacity for cell-to-cell spread and hence smaller plaque sizes. Infectious laryngotracheitis virus (ILTV), or Gallid herpesvirus 1, is an alphaherpesvirus that causes respiratory disease in chickens. The roles of gI and gE in ILTV have not been investigated previously. In this study, a glycoprotein I and glycoprotein E deletion mutant of ILTV (gI/gE-ve ILTV) was generated by replacing the region of the ILTV genome coding for the adjacent gI and gE genes with the gene for enhanced green fluorescent protein (eGFP). This gI/E-ve ILTV was readily propagated in cell culture in the presence of wildtype ILTV (wt ILTV). However, in the absence of wt ILTV the propagation of gI/gE-ve ILTV was severely impaired. Infection of permissive cell cultures with gI/gE-ve ILTV failed to produce plaques but single infected cells could be identified by fluorescence microscopy. This suggests that gI/gE has a more significant role in the cell-to-cell spread of ILTV in vitro than in many oth...Continue Reading

References

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Citations

May 31, 2013·Avian Pathology : Journal of the W.V.P.A·Mauricio J C CoppoJoanne M Devlin
May 21, 2011·Virology Journal·Lijuan LiXiaoyue Chen
Dec 27, 2011·Comparative Immunology, Microbiology and Infectious Diseases·Alireza MahmoudianGlenn F Browning
Aug 22, 2014·Avian Pathology : Journal of the W.V.P.A·Vishwanatha R A P ReddyHans J Nauwynck
Feb 14, 2007·Veterinary Research·Walter FuchsThomas C Mettenleiter
Sep 12, 2006·The Journal of General Virology·J M DevlinJ R Gilkerson

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