A Glyoxalase-1 Knockdown Does Not Have Major Short Term Effects on Energy Expenditure and Atherosclerosis in Mice

Journal of Diabetes Research
Markus WortmannSusanne Dihlmann

Abstract

Objective. Glyoxalase-1 is an enzyme detoxifying methylglyoxal (MG). MG is a potent precursor of advanced glycation endproducts which are regarded to be a key player in micro- and macrovascular damage. Yet, the role of Glo1 in atherosclerosis remains unclear. In this study, the effect of Glo1 on mouse metabolism and atherosclerosis is evaluated. Methods. Glo1 knockdown mice were fed a high fat or a standard diet for 10 weeks. Body weight and composition were investigated by Echo MRI. The PhenoMaster system was used to measure the energy expenditure. To evaluate the impact of Glo1 on atherosclerosis, Glo1(KD) mice were crossed with ApoE-knockout mice and fed a high fat diet for 14 weeks. Results. Glo1 activity was significantly reduced in heart, liver, and kidney lysates derived from Glo1(KD) mice. Yet, there was no increase in methylglyoxal-derived AGEs in all organs analyzed. The Glo1 knockdown did not affect body weight or body composition. Metabolic studies via indirect calorimetry did not show significant effects on energy expenditure. Glo1(KD) mice crossed to ApoE(-/-) mice did not show enhanced formation of atherosclerosis. Conclusion. A Glo1 knockdown does not have major short term effects on the energy expenditure or th...Continue Reading

References

Oct 25, 2000·American Journal of Physiology. Heart and Circulatory Physiology·C J HartleyY X Wang
Apr 16, 2003·Circulation·Ronald M A HenryUNKNOWN Hoorn Study
Dec 15, 2007·Annals of the New York Academy of Sciences·Katrien H J GaensCasper G Schalkwijk
Sep 24, 2008·The Journal of Experimental Medicine·Assam El-OstaMichael Brownlee
Feb 16, 2011·Seminars in Cell & Developmental Biology·Mingzhan XuePaul J Thornalley
Dec 30, 2011·Nature Methods·Matthias H TschöpEric Ravussin
Jun 26, 2013·Gerontology·Thomas H FlemingPeter P Nawroth
Mar 22, 2014·Biochemical Society Transactions·Markus WortmannSusanne Dihlmann
Jul 18, 2014·PLoS Genetics·Ville-Petteri MäkinenThemistocles L Assimes
Feb 11, 2015·Biochemical and Biophysical Research Communications·Naila Rabbani, Paul J Thornalley

❮ Previous
Next ❯

Citations

May 12, 2016·Biochimica Et Biophysica Acta·Raquel López-DíezAnn Marie Schmidt
Jun 18, 2017·Biochemical and Biophysical Research Communications·Sumi JangChankyu Park
Oct 7, 2020·Antioxidants·Jakob MorgensternThomas Fleming

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
PCR
X-ray
ELISA
nuclear magnetic resonance

Software Mentioned

SPSS Statistics
SigmaStat
Echo
PhenoMaster
ImageJ

Related Concepts

Related Feeds

Atherosclerosis Disease Progression

Atherosclerosis is the buildup of plaque on artery walls, causing stenosis which can eventually lead to clinically apparent cardiovascular disease. Find the latest research on atherosclerosis disease progression here.

ApoE Phenotypes

Apolipoprotein E (APOE) is a protein involved in fat metabolism and associated with the pathogenesis of Alzheimer's disease and cardiovascular disease. Here is the latest research on APOE phenotypes.

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.