Abstract
Objective. Glyoxalase-1 is an enzyme detoxifying methylglyoxal (MG). MG is a potent precursor of advanced glycation endproducts which are regarded to be a key player in micro- and macrovascular damage. Yet, the role of Glo1 in atherosclerosis remains unclear. In this study, the effect of Glo1 on mouse metabolism and atherosclerosis is evaluated. Methods. Glo1 knockdown mice were fed a high fat or a standard diet for 10 weeks. Body weight and composition were investigated by Echo MRI. The PhenoMaster system was used to measure the energy expenditure. To evaluate the impact of Glo1 on atherosclerosis, Glo1(KD) mice were crossed with ApoE-knockout mice and fed a high fat diet for 14 weeks. Results. Glo1 activity was significantly reduced in heart, liver, and kidney lysates derived from Glo1(KD) mice. Yet, there was no increase in methylglyoxal-derived AGEs in all organs analyzed. The Glo1 knockdown did not affect body weight or body composition. Metabolic studies via indirect calorimetry did not show significant effects on energy expenditure. Glo1(KD) mice crossed to ApoE(-/-) mice did not show enhanced formation of atherosclerosis. Conclusion. A Glo1 knockdown does not have major short term effects on the energy expenditure or th...Continue Reading
References
Oct 25, 2000·American Journal of Physiology. Heart and Circulatory Physiology·C J HartleyY X Wang
Feb 27, 2003·Chemico-biological Interactions·David L Vander Jagt, Lucy A Hunsaker
Apr 16, 2003·Circulation·Ronald M A HenryUNKNOWN Hoorn Study
Nov 25, 2004·Diabetes Care·Maximilian von EynattenKlaus A Dugi
Jul 30, 2005·Hearing Research·Yunkai GuoTai-June Yoo
Dec 15, 2007·Annals of the New York Academy of Sciences·Katrien H J GaensCasper G Schalkwijk
Sep 24, 2008·The Journal of Experimental Medicine·Assam El-OstaMichael Brownlee
Feb 16, 2011·Seminars in Cell & Developmental Biology·Mingzhan XuePaul J Thornalley
Dec 7, 2011·Diabetologia·A K BernerA W Stitt
Dec 30, 2011·Nature Methods·Matthias H TschöpEric Ravussin
Oct 23, 2012·Neurochemistry International·Robert C LyonElizabeth M Ellis
Jun 26, 2013·Gerontology·Thomas H FlemingPeter P Nawroth
Oct 16, 2013·European Heart Journal·Nordin M J HanssenCasper G Schalkwijk
Oct 29, 2013·Diabetologia·Olaf BrouwersCasper G Schalkwijk
Mar 22, 2014·Biochemical Society Transactions·Markus WortmannSusanne Dihlmann
May 9, 2014·Diabetes·Chris TikellisMerlin C Thomas
Jun 13, 2014·Physiological Reports·Michèle GeoffrionRoss W Milne
Jul 18, 2014·PLoS Genetics·Ville-Petteri MäkinenThemistocles L Assimes
Aug 21, 2014·Cardiovascular Research·Nordin M J HanssenCasper G Schalkwijk
Feb 11, 2015·Biochemical and Biophysical Research Communications·Naila Rabbani, Paul J Thornalley
Citations
May 12, 2016·Biochimica Et Biophysica Acta·Raquel López-DíezAnn Marie Schmidt
Jun 18, 2017·Biochemical and Biophysical Research Communications·Sumi JangChankyu Park
Aug 25, 2016·Clinical Science·Naila RabbaniPaul J Thornalley
Sep 5, 2019·Frontiers in Oncology·Maurice MichelAlexander Zipprich
Oct 7, 2020·Antioxidants·Jakob MorgensternThomas Fleming
Dec 2, 2020·Antioxidants·Jan ŠilhavýMichal Pravenec