A gold nanoparticle-based lateral flow immunosensor for ultrasensitive detection of tetrodotoxin

The Analyst
Yue LiChuanlai Xu

Abstract

Tetrodotoxin (TTX) is a potent marine neurotoxin. Its frequent detection and widespread distribution pose a substantial health risk to consumers. The work presented herein describes the development of a lateral flow immunosensor based on a gold nanoparticle-labeled monoclonal antibody probe, which allows the affinity reaction of antigen-antibody and amplifies the reaction signal. The immunosensor assay was found to provide a visible limit of detection (vLOD) of 10 μg kg-1 in crucian and clam matrices without time-consuming pretreatment. Spiked samples were analyzed using the immunosensor and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The immunosensor gave average intra-assay recoveries of 84.0-103.9%, inter-assay recoveries of 76.1-105% for the crucian matrix and intra-assay recoveries of 78.5-93.0%, inter-assay recoveries of 74.8-85.7% for the clam matrix. The results obtained using the immunosensor and LC-MS/MS were in good agreement. The immunosensor test could be completed within 10 min, offering a convenient option or complementarity to the traditional methods of TTX detection.

References

Jul 11, 1975·Science·Y H KimF A Mosher
Dec 8, 2004·Toxicon : Official Journal of the International Society on Toxinology·Osmindo R PiresCarlos A Schwartz
Jul 15, 2009·Toxicon : Official Journal of the International Society on Toxinology·Mari Yotsu-Yamashita, Eiko Tateki
Feb 3, 2012·Chemical Reviews·Krishnendu SahaVincent M Rotello
May 1, 2012·Analytical and Bioanalytical Chemistry·Laura AnfossiGianfranco Giraudi
Dec 4, 2013·Journal of Immunoassay & Immunochemistry·Aree ThattiyaphongLaddawan Rojanapantip
Feb 26, 2014·Toxins·Vaishali BaneAmbrose Furey
Aug 15, 2014·Analytical Chemistry·Betsy Jean YakesStacey L DeGrasse
Jan 24, 2015·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·A D TurnerC Baker-Austin
Oct 12, 2016·Current Biology : CB·Max N LorentzStefan Lötters
Feb 18, 2017·Marine Drugs·Penelope AjaniShauna A Murray
Oct 7, 2017·Bioconjugate Chemistry·Nadezhda A ByzovaBoris B Dzantiev

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Methods Mentioned

BETA
enzyme-linked
surface plasmon resonance
ic-ELISA
biosensor

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