A gold nanoparticle-based semi-quantitative and quantitative ultrasensitive paper sensor for the detection of twenty mycotoxins

Nanoscale
Dezhao KongChuanlai Xu

Abstract

A semi-quantitative and quantitative multi-immunochromatographic (ICA) strip detection assay was developed for the simultaneous detection of twenty types of mycotoxins from five classes, including zearalenones (ZEAs), deoxynivalenols (DONs), T-2 toxins (T-2s), aflatoxins (AFs), and fumonisins (FBs), in cereal food samples. Sensitive and specific monoclonal antibodies were selected for this assay. The semi-quantitative results were obtained within 20 min by the naked eye, with visual limits of detection for ZEAs, DONs, T-2s, AFs and FBs of 0.1-0.5, 2.5-250, 0.5-1, 0.25-1 and 2.5-10 μg kg(-1), and cut-off values of 0.25-1, 5-500, 1-10, 0.5-2.5 and 5-25 μg kg(-1), respectively. The quantitative results were obtained using a hand-held strip scan reader, with the calculated limits of detection for ZEAs, DONs, T-2s, AFs and FBs of 0.04-0.17, 0.06-49, 0.15-0.22, 0.056-0.49 and 0.53-1.05 μg kg(-1), respectively. The analytical results of spiked samples were in accordance with the accurate content in the simultaneous detection analysis. This newly developed ICA strip assay is suitable for the on-site detection and rapid initial screening of mycotoxins in cereal samples, facilitating both semi-quantitative and quantitative determination.

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Citations

Jan 11, 2017·Critical Reviews in Food Science and Nutrition·Pranav TripathiSeema Nara
Feb 6, 2017·Biotechnology Letters·Alexandr E UrusovBoris B Dzantiev
Jun 29, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Lu WeiShi-Gang Sun
Feb 1, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Guilan ZhangAiliang Chen
Jan 13, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kevin A KirkSilvana Andreescu
Aug 21, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xianlu LeiChuanlai Xu
Sep 19, 2018·Nanomaterials·Pavel HorkyJiri Skladanka
Sep 27, 2019·Frontiers in Plant Science·Zitong GaoJianping Han
Sep 16, 2020·Analytical Methods : Advancing Methods and Applications·Jie LiuChuanlai Xu
Sep 16, 2020·Analytical Methods : Advancing Methods and Applications·Zhijun GuoHansong Yu
Oct 22, 2020·Analytical Methods : Advancing Methods and Applications·Lingling GuoChuanlai Xu
Feb 4, 2021·The Analyst·Linxia WuDizhe Wei
Feb 27, 2021·Journal of Immunoassay & Immunochemistry·Younes SbihiAbdelghani Iddar
May 7, 2021·Critical Reviews in Food Science and Nutrition·Liu WangXiahong Xu

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