A gradient of TFF3 (trefoil factor family 3) peptide synthesis within the normal human gastric mucosa

Cell and Tissue Research
Irina KouznetsovaWerner Hoffmann

Abstract

TFF3 is a member of the trefoil factor family (TFF) peptides, which enhance the surface integrity of mucous epithelia, and is typically secreted by intestinal goblet cells together with the mucin MUC2. Reports of the expression of TFF3 within the normal human stomach are contradictory and the precise localisation of TFF3 is unknown. We have mapped the human gastric mucosa by reverse transcription/polymerase chain reaction and Western blot analysis and by immunohistochemistry. Small amounts of TFF3 were detectable in the oxyntic mucosa of the corpus, whereas TFF3 synthesis increased sharply distal to the corpus-antrum transitional zone. TFF3 secretion was demonstrated in the antrum, the pyloric region and the proximal duodenum. High TFF1 levels were present in all regions of the gastric mucosa including the corpus, whereas the proximal duodenum was nearly devoid of TFF1. Immunohistochemistry localised TFF3 to antral and pyloric surface mucous cells. In the antrum, an increasing concentration gradient was found towards cells deeper in the isthmus. TFF1 was confined to superficial pit cells in the antrum. TFF3 was also present in specific layers of the laminated mucous gel of the antral and pyloric regions and variable concentrati...Continue Reading

Citations

May 24, 2005·Cell and Tissue Research·Friedrich PaulsenMichael Tsokos
Aug 27, 2005·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Mirela Baus-LoncarTuncay Kayademir
Nov 6, 2007·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Irina KouznetsovaWerner Hoffmann
Sep 23, 2006·American Journal of Respiratory Cell and Molecular Biology·Irina KouznetsovaWerner Hoffmann
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Nov 28, 2019·International Journal of Molecular Sciences·Franziska HeuerWerner Hoffmann
Jan 23, 2020·International Journal of Molecular Sciences·Eva B ZnalesniakWerner Hoffmann

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