A graft-based chemotherapy method for screening effective molecules and rescuing huanglongbing-affected citrus plants

Phytopathology
Muqing ZhangYongping Duan

Abstract

Huanglongbing (HLB) is the most devastating disease of citrus. The global citrus industry is in urgent need of effective chemical treatments for HLB control because of its rapid spreading worldwide. Due to the fastidious nature of the pathogens, and the poor permissibility of citrus leaf surfaces, effective screening of chemicals for the HLB control can be challenging. In this study, we developed a graft-based chemotherapy method to rapidly screen potential HLB-controlling chemical compounds. In addition, we improved transmission efficiency by using the best HLB-affected scion-rootstock combination, and demonstrated the HLB bacterial titer was the critical factor in transmission. The HLB-affected lemon scions had a high titer of HLB bacterium, survival rate (83.3%), and pathogen transmission rate (59.9%). Trifoliate, a widely used commercial rootstock, had the highest survival rate (>70.0%) compared with grapefruit (52.6%) and sour orange (50.4%). Using this method, we confirmed a mixture of penicillin and streptomycin was the most effective compounds in eliminating the HLB bacterium from the HLB-affected scions, and in successfully rescuing severely HLB-affected citrus germplasms. These findings are useful not only for chemica...Continue Reading

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Citations

Feb 15, 2013·Standards in Genomic Sciences·Michael T LeonardEric W Triplett
Dec 11, 2014·Pest Management Science·Dhana Raj Boina, Jeffrey R Bloomquist
Jul 28, 2016·PloS One·Federico MartinelliAbhaya M Dandekar
Dec 3, 2017·Microbial Ecology·Shahzad MunirYueqiu He
Jun 7, 2018·Horticulture Research·Fang DingYongping Duan
Feb 16, 2019·Frontiers in Plant Science·Muqing ZhangYongping Duan
Oct 11, 2020·Antibiotics·Nabil KillinyChristopher I Vincent
Oct 18, 2020·Antibiotics·Faraj HijazNabil Killiny

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