A graphene-based fluorescent nanoprobe for simultaneous monitoring of miRNA and mRNA in living cells

Nanoscale
Wei PanBo Tang

Abstract

Since microRNA-21 (miR-21) and protein programmed cell death 4 (PDCD4) are implicated in tumor progression, simultaneous monitoring of the dynamic variation of miR-21 and PDCD4 mRNA in living cells is of great interest for understanding their relationship in the pathology of the disease. Herein, a versatile nanoprobe based on graphene oxide (GO) and DNA was prepared which can simultaneously monitor and visualize miR-21 and PDCD4 mRNA in living cells. In vitro experiments demonstrate that the nanoprobe exhibits outstanding selectivity and high sensitivity towards miR-21 and PDCD4 mRNA. Moreover, by exploiting the DNA/GO sensing platform, simultaneous imaging of miR-21 and PDCD4 mRNA in MCF-7 human breast cancer cells has been realized. More significantly, an inverse correlation between miR-21 and PDCD4 mRNA was verified by fluorescence confocal imaging and RT-PCR results, indicating the distinguishing ability of the nanoprobe at various expression levels of RNAs. The current strategy provides a promising tool to implement the detection of miR-21 and PDCD4 mRNA in cancer cells and can be expected to be applied for other biomolecular sensing.

References

May 1, 2001·Nature Medicine·K L NathansonK N Nathanson
Oct 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Diana P BratuSanjay Tyagi
Apr 16, 2004·Nucleic Acids Research·Philip J SantangeloGang Bao
Jul 19, 2005·Cancer Research·Jennifer A ChanKenneth S Kosik
Sep 6, 2005·Science·Phillip D Zamore, Benjamin Haley
Mar 25, 2006·Nature Reviews. Cancer·Aurora Esquela-Kerscher, Frank J Slack
Sep 13, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Claudia RoldoCarlo M Croce
Nov 10, 2007·The Journal of Biological Chemistry·Lisa B FrankelAnders H Lund
Nov 24, 2007·Journal of the American Chemical Society·Dwight S SeferosChad A Mirkin
Feb 14, 2008·Cell Research·Shuomin ZhuYin-Yuan Mo
Mar 7, 2009·Nucleic Acids Research·Nalinie S WickramasingheCarolyn M Klinge
Sep 5, 2009·Angewandte Chemie·Shiping SongChunhai Fan
Apr 27, 2010·Journal of Dermatological Science·John R ZibertLone Skov
Feb 8, 2011·CA: a Cancer Journal for Clinicians·Ahmedin JemalDavid Forman
Jul 29, 2011·Chemical Society Reviews·Xiao HuangHua Zhang
Mar 14, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Flavie SicardPierre Cordelier
Jul 31, 2013·Current Pharmaceutical Design·Honglei TuYongchang Wei
Apr 12, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Pedro M CostaMaria C Pedroso de Lima

❮ Previous
Next ❯

Citations

Jul 5, 2019·Advanced Materials·Devleena SamantaChad A Mirkin
Dec 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Dinggeng HeHung-Wing Li
Mar 14, 2021·Colloids and Surfaces. B, Biointerfaces·Zhongying GongJingkun Bai
Apr 20, 2021·Analytical Methods : Advancing Methods and Applications·Xiaojuan AiChangbei Ma
Jul 17, 2021·Journal of Nanobiotechnology·Guangman CuiGuangyu Yao
Jun 24, 2020·Journal of the American Chemical Society·Sasha B EbrahimiChad A Mirkin
Nov 22, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Jie YangYi-Tao Long
Jan 19, 2021·ACS Biomaterials Science & Engineering·Zahra Mohammadpour, Keivan Majidzadeh-A

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence resonance
fluorescence imaging
FRET
transmission electron microscopy
Fluorescence
PCR
AFM
biosensors
biosensor
fluorescence microscopy

Related Concepts