A Graphene-PEDOT:PSS Modified Paper-Based Aptasensor for Electrochemical Impedance Spectroscopy Detection of Tumor Marker

Sensors
Yi-Kuang YenYa-Shin Yeh

Abstract

A graphene and poly (3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) modified conductive paper-based electrochemical impedance spectroscopy (EIS) aptasensor has been successfully fabricated by a simple and continuous coating process. A graphene/PEDOT:PSS modified paper electrode forms the nanocomposite providing a conductive and sensitive substrate for further aptamer functionalization of the biosensor. This low-cost paper-based aptasensor exhibits its sensitivity to carcinoembryonic antigens (CEA) in standard buffer solutions and human serum samples in a linear range of 0.77-14 ng·mL-1. The limit of detection (LOD) is found to be 0.45 ng·mL-1 and 1.06 ng·mL-1 for CEA in both samples, separately. This aptamer-based sensing device was also evaluated and received a good correlation with the immunoassay detection method. The proposed paper-based aptasensor has demonstrated its potential as a rapid simple point-of-care analytical platform for early cancer diagnosis in less developed areas where manufacturing facilities, analytical instruments, and trained specialists are limited.

References

Feb 14, 2006·Biosensors & Bioelectronics·Steven A SoperDavid Wilson
Dec 13, 2006·Nature·Mickey UrdeaDeborah C Hay Burgess
Jan 8, 2008·Electroanalysis·Jonathan S Daniels, Nader Pourmand
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Citations

Sep 18, 2020·Analytical and Bioanalytical Chemistry·Mandana AmiriReyhaneh Sadat Saberi
Oct 7, 2020·Biosensors & Bioelectronics·Tao MingXinxia Cai
Jan 28, 2021·Sensors·Kamila MaleckaElena E Ferapontova
Apr 22, 2021·Lab on a Chip·Wen-Chin LeeLung-Ming Fu

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Methods Mentioned

BETA
Aptasensor

Software Mentioned

PSTrace

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