A group II intron encodes a functional LAGLIDADG homing endonuclease and self-splices under moderate temperature and ionic conditions.

RNA
Sahra-Taylor MullineuxGeorg Hausner

Abstract

A group II intron encoding a protein belonging to the LAGLIDADG family of homing endonucleases was identified in the mitochondrial rns gene of the filamentous fungus Leptographium truncatum, and the catalytic activities of both the intron and its encoded protein were characterized. A model of the RNA secondary structure indicates that the intron is a member of the IIB1 subclass and the open reading frame is inserted in ribozyme domain III. In vitro assays carried out with two versions of the intron, one in which the open reading frame was removed and the other in which it was present, demonstrate that both versions of the intron readily self-splice at 37 degrees C and at a concentration of MgCl(2) as low as 6 mM. The open reading frame encodes a functional LAGLIDADG homing endonuclease that cleaves 2 (top strand) and 6 (bottom strand) nucleotides (nt) upstream of the intron insertion site, generating 4 nt 3' OH overhangs. In vitro splicing assays carried out in the absence and presence of the intron-encoded protein indicate that the protein does not enhance intron splicing, and RNA-binding assays show that the protein does not appear to bind to the intron RNA precursor transcript. These findings raise intriguing questions conce...Continue Reading

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Citations

Feb 11, 2014·Fungal Genetics and Biology : FG & B·Tuhin Kumar Guha, Georg Hausner
Apr 13, 2011·BMC Biology·David R EdgellMarlene Belfort
Aug 16, 2012·Genome Génome / Conseil National De Recherches Canada·Mohamed Hafez, Georg Hausner
May 12, 2015·Mobile DNA·Steven Zimmerly, Cameron Semper
Jul 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ryo TakeuchiBarry L Stoddard
Apr 18, 2017·Canadian Journal of Microbiology·Iman M BiltoGeorg Hausner
Jun 1, 2018·Genome Biology and Evolution·Wilson X GuilloryAndrew J Alverson
Nov 9, 2019·Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis·Abdullah ZubaerGeorg Hausner
Nov 7, 2019·Yeast·Bernard Dujon
Oct 31, 2020·Archives of Insect Biochemistry and Physiology·Travis GlareMurray Cox
Jun 25, 2015·Microbiology Spectrum·Alan M Lambowitz, Marlene Belfort
Jun 22, 2021·Frontiers in Microbiology·Chase G MayersGeorg Hausner
Feb 11, 2021·Canadian Journal of Microbiology·Alvan Wai, Georg Hausner
Aug 28, 2021·Cells·Jigeesha Mukhopadhyay, Georg Hausner

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