A Group of Novel Serum Diagnostic Biomarkers for Multidrug-Resistant Tuberculosis by iTRAQ-2D LC-MS/MS and Solexa Sequencing

International Journal of Biological Sciences
Chong WangJi-Cheng Li

Abstract

The epidemic of pulmonary tuberculosis (TB), especially multidrug-resistance tuberculosis (MDR-TB) presented a major challenge for TB treatment today. We performed iTRAQ labeling coupled with two-dimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS) and Solexa sequencing among MDR-TB patients, drug-sensitive tuberculosis (DS-TB) patients, and healthy controls. A total of 50 differentially expressed proteins and 43 differentially expressed miRNAs (fold change >1.50 or <0.60, P<0.05) were identified in the MDR-TB patients compared to both DS-TB patients and healthy controls. We found that 22.00% of differentially expressed proteins and 32.56% of differentially expressed miRNAs were related, and could construct a network mainly in complement and coagulation cascades. Significant differences in CD44 antigen (CD44), coagulation factor XI (F11), kininogen-1 (KNG1), miR-4433b-5p, miR-424-5p, and miR-199b-5p were found among MDR-TB patients, DS-TB patients and healthy controls (P<0.05) by enzyme-linked immunosorbent assay (ELISA) and SYBR green qRT-PCR validation. A strong negative correlation, consistent with the target gene prediction, was found between miR-199b-5p and KNG1 (r=-0.232, P=0.017). Moreover, we establi...Continue Reading

Citations

Nov 10, 2017·Mass Spectrometry Reviews·Robert MoulderRiitta Lahesmaa
Apr 9, 2019·Bioinformatics·W Jenny ShiKaterina Kechris
Aug 12, 2020·JCI Insight·Diana J Garay-BaqueroPaul Elkington
Apr 14, 2018·Frontiers in Microbiology·Naveed SabirXiangmei Zhou
Sep 30, 2020·Cells·Alessandro SinigagliaLuisa Barzon
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Feb 23, 2019·BioMed Research International·Yan Yang, Jianqing Wu
Oct 2, 2019·Frontiers in Cellular and Infection Microbiology·Liping PanZongde Zhang
Feb 13, 2020·Experimental Biology and Medicine·Huai HuangJi-Cheng Li
Jan 8, 2020·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Lin GanJi-Cheng Li
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Jan 27, 2021·Drug Discovery Today·Amit Kumar SinghShripad A Patil

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Methods Mentioned

BETA
PCRs
ELISA

Software Mentioned

BLAST
MedCalc
Cytoscape
Blast2GO PRO
Biomarker Pattern Software ( BPS
TIGR MultiExperiment Viewer
ProteinPilotTM
STRING
SPSS
BLAST search

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