A G.U base pair in the eukaryotic selenocysteine tRNA is important for interaction with SePF, the putative selenocysteine-specific elongation factor

FEBS Letters
T MizutaniK Yamada

Abstract

In Escherichia coli, selenocysteine biosynthesis and incorporation into selenoproteins requires the action of four gene products, including the specialized selenocysteine tRNA(Sec) and elongation factor SELB, different from the universal EF-Tu. In this regard, the situation is less clear in eukaryotes, but we previously reported the existence of SePF, a putative SELB homologue. The secondary structure of the tRNA(Sec) differs slightly in eukaryotes, due to a change in the lengths of several stems. Two non-Watson-Crick base pairs, G5a x U67b and U6 x U67, reside in the acceptor stem and are conserved in the course of evolution. Since it has already been reported that changing them to Watson-Crick base pairs did not affect the serylation or selenylation levels of tRNA(Sec), we asked whether these non-Watson-Crick base pairs are required for the interaction with SePF. To this end, tRNA(Sec) variants carrying Watson-Crick changes at these positions were tested for their ability to maintain the interaction with SePF. In these assays, the tRNA(Sec)-SePF interaction was determined by the protective action it confers against hydrolysis of the amino acid ester bond, under basic conditions. All the changes introduced at U6 x U67 did not ...Continue Reading

References

Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·C GelpiJ L Rodriguez-Sanchez
Jun 15, 1992·The Biochemical Journal·T MizutaniT Totsuka
Sep 2, 1991·FEBS Letters·T MizutaniK Yamada
May 1, 1990·Molecular and Cellular Biology·B J LeeD Hatfield
Feb 15, 1990·The Biochemical Journal·T HitakaT Totsuka
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B L Seong, U L RajBhandary
Sep 11, 1987·Nucleic Acids Research·Y SukenagaK Takagi
Nov 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·D Hatfield, F H Portugal
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·R Stapulionis, M P Deutscher
Oct 18, 1996·Cell·Y NakamuraL A Isaksson
Oct 18, 1996·Journal of Molecular Biology·R AmbergH J Gross
Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·I Y KimT C Stadtman
Jan 1, 1997·Annual Review of Biochemistry·S R Sprang
Oct 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·A W CurnowD Söll
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·L BénardC Portier

❮ Previous
Next ❯

Citations

Sep 25, 2001·BioFactors·P R Copeland, D M Driscoll
Jul 2, 2008·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Takaharu MizutaniM Shahidzzman
Apr 11, 2009·Biochimica Et Biophysica Acta·Leopold Flohé
Jan 11, 2003·Natural Product Reports·Marc BirringerLeopold Flohé
Nov 17, 2020·Frontiers in Microbiology·Natalie KrahnDieter Söll
Jun 2, 2006·Biochimie·C Allmang, A Krol

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.