A Guide to Native Mass Spectrometry to determine complex interactomes of molecular machines.

The FEBS Journal
Rita PuglisiAnnalisa Pastore

Abstract

Native mass spectrometry is an emerging technique in biology that gives the possibility to study noncovalently bound complexes with high sensitivity and accuracy. It thus allows the characterization of macromolecular assemblies, assessing their mass and stoichiometries and mapping the interacting surfaces. In this review, we discuss the application of native mass spectrometry to dynamic molecular machines based on multiple weak interactions. In the study of these machines, it is crucial to understand which and under which conditions various complexes form at any time point. We focus on the specific example of the iron-sulfur cluster biogenesis machine because this is an archetype of a dynamic machine that requires very specific and demanding experimental conditions, such as anaerobicity and the need of retaining the fold of marginally folded proteins. We describe the advantages, challenges and current limitations of the technique by providing examples from our own experience and suggesting possible future solutions.

References

Apr 1, 1991·Rapid Communications in Mass Spectrometry : RCM·V Katta, B T Chait
Mar 16, 1990·Biochemical and Biophysical Research Communications·S K ChowdhuryB T Chait
Feb 27, 1999·Current Opinion in Structural Biology·A A Rostom, C V Robinson
Oct 6, 1999·Current Opinion in Chemical Biology·A M Last, C V Robinson
Apr 27, 2000·The Journal of Biological Chemistry·C T Lauhon, R Kambampati
Sep 29, 2001·The Journal of Biological Chemistry·H D UrbinaL E Vickery
Jun 28, 2003·FEMS Microbiology Reviews·Jennifer S CavetNigel J Robinson
Aug 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·J Andrew AquilinaCarol V Robinson
Jul 28, 2004·Journal of Molecular Biology·Fadi Bou-AbdallahN Dennis Chasteen
Jun 1, 2005·Proceedings of the National Academy of Sciences of the United States of America·Leopold L IlagCarol V Robinson
Apr 4, 2006·Analytical Chemistry·Alexander MakarovStevan Horning
Nov 1, 2006·Analytical Chemistry·Robert H H van den HeuvelAlbert J R Heck
Apr 5, 2007·Nature Protocols·Helena Hernández, Carol V Robinson
Jun 17, 2008·Science·Nelson P BarreraCarol V Robinson
Mar 24, 2009·Nature Structural & Molecular Biology·Salvatore AdinolfiAnnalisa Pastore
Jan 12, 2010·Biochemistry·Przemysław GrelaMarek Tchórzewski
Apr 27, 2010·Molecular & Cellular Proteomics : MCP·Glen K ShoemakerAlbert J R Heck
May 18, 2010·Protein Expression and Purification·Filippo PrischiAnnalisa Pastore
Jul 28, 2011·Angewandte Chemie·Charlotte Uetrecht, Albert J R Heck
Jan 24, 2012·Journal of Structural Biology·Jessica Z BereszczakAlbert J R Heck
Jan 10, 2013·Biochimica Et Biophysica Acta·Béatrice RocheFrédéric Barras
Mar 2, 2013·Angewandte Chemie·Joost SnijderAlbert J R Heck
Apr 17, 2013·Proceedings of the National Academy of Sciences of the United States of America·Andrey DyachenkoMichal Sharon
Jul 11, 2013·The Journal of Biological Chemistry·Robert YanAnnalisa Pastore
Jul 24, 2013·Journal of Neurochemistry·Annalisa Pastore, Helene Puccio
Dec 24, 2013·Structure·David VeeslerJohn E Johnson
May 9, 2014·Journal of the American Chemical Society·Jin Hae KimJohn L Markley
Jun 12, 2014·Protein Science : a Publication of the Protein Society·Clara IannuzziAnnalisa Pastore
Jul 12, 2014·Annual Review of Analytical Chemistry·Joost Snijder, Albert J R Heck

❮ Previous
Next ❯

Citations

Feb 24, 2021·Emerging Topics in Life Sciences·Amanda L Smythers, Leslie M Hicks
May 27, 2021·Dalton Transactions : an International Journal of Inorganic Chemistry·Jason C CrackNick E Le Brun

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Accounts of Chemical Research
J F Stoddart
Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry
Wesley R BrowneDahui Hu
Biophysics Reviews
Robert Cross, Claudia Veigel
Frontier Nursing Service Quarterly Bulletin
Current Opinion in Chemical Biology
Stephen J Benkovic, Kevin D Raney
© 2021 Meta ULC. All rights reserved