A Guide to Studying Human Hair Follicle Cycling In Vivo

The Journal of Investigative Dermatology
Ji Won OhMaksim V Plikus

Abstract

Hair follicles (HFs) undergo lifelong cyclical transformations, progressing through stages of rapid growth (anagen), regression (catagen), and relative "quiescence" (telogen). Given that HF cycling abnormalities underlie many human hair growth disorders, the accurate classification of individual cycle stages within skin biopsies is clinically important and essential for hair research. For preclinical human hair research purposes, human scalp skin can be xenografted onto immunocompromised mice to study human HF cycling and manipulate long-lasting anagen in vivo. Although available for mice, a comprehensive guide on how to recognize different human hair cycle stages in vivo is lacking. In this article, we present such a guide, which uses objective, well-defined, and reproducible criteria, and integrates simple morphological indicators with advanced, (immuno)-histochemical markers. This guide also characterizes human HF cycling in xenografts and highlights the utility of this model for in vivo hair research. Detailed schematic drawings and representative micrographs provide examples of how best to identify human HF stages, even in suboptimally sectioned tissue, and practical recommendations are given for designing human-on-mouse h...Continue Reading

Citations

Feb 27, 2016·F1000Research·Bruno A Bernard
May 24, 2016·International Journal of Cosmetic Science·M MaurerF Siebenhaar
Feb 22, 2017·Dermatology and Therapy·María Abril Martínez-VelascoAntonella Tosti
Sep 28, 2017·The Oncologist·Christopher John DunnillNikolaos Theodoros Georgopoulos
Aug 30, 2018·Stem Cells International·Agnieszka Owczarczyk-SaczonekJoanna Wojtkiewicz
Sep 4, 2019·American Journal of Human Biology : the Official Journal of the Human Biology Council·Sandra L KochNina G Jablonski
Jan 7, 2020·Experimental Dermatology·Etienne C E Wang, Claire A Higgins
Jan 28, 2020·International Journal of Medicinal Chemistry·H RambwawasvikaL Gwatidzo
Sep 27, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kristelle HughesPhila Raharivelomanana
Feb 5, 2016·Experimental Dermatology·Stefanie Heilmann-HeimbachMarkus M Nöthen
Apr 21, 2016·Experimental Dermatology·Talveen S PurbaRalf Paus
Mar 8, 2017·Experimental Dermatology·Bruno A Bernard
Jun 20, 2017·The British Journal of Dermatology·C DessiniotiA J Stratigos
Apr 20, 2018·Experimental Dermatology·Andrey A Panteleyev
Jan 13, 2018·Nature Reviews. Endocrinology·Christian F Guerrero-Juarez, Maksim V Plikus
Jun 14, 2018·The British Journal of Dermatology·M AlamF Jimenez
Mar 20, 2019·The EMBO Journal·Bénédicte OulèsFiona M Watt
Mar 14, 2019·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Ana Rita CastroElsa Logarinho
Mar 9, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kinga Fanni TóthAttila Oláh
Apr 26, 2019·Experimental Dermatology·Argentina LeonGil Yosipovitch
Nov 27, 2019·Experimental Dermatology·Yuval RamotRalf Paus
Jul 2, 2019·Experimental Dermatology·Megan A PalmerIain S Haslam
Jul 19, 2020·Experimental Dermatology·Marta BertoliniRalf Paus
Oct 11, 2017·Mathematical Medicine and Biology : a Journal of the IMA·Atanaska DobrevaN G Cogan
Jun 9, 2017·The Journal of Dermatology·Jiang JiMei Bigliardi-Qi
Dec 22, 2017·Anais Da Academia Brasileira De Ciências·Chun HouWeijin Hong
Sep 13, 2019·EMBO Molecular Medicine·Talveen S PurbaRalf Paus
Jan 29, 2020·Experimental Dermatology·Jean-Luc CarréRalf Paus
Mar 4, 2020·Journal of Applied Toxicology : JAT·Christopher DaviesJoanne Morrissey
Sep 24, 2020·Biological Reviews of the Cambridge Philosophical Society·James D B O'SullivanRalf Paus

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Methods Mentioned

BETA
biopsies
xenograft
xenografting
xenografts

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