A guide to super-resolution fluorescence microscopy.

The Journal of Cell Biology
Lothar SchermellehHeinrich Leonhardt

Abstract

For centuries, cell biology has been based on light microscopy and at the same time been limited by its optical resolution. However, several new technologies have been developed recently that bypass this limit. These new super-resolution technologies are either based on tailored illumination, nonlinear fluorophore responses, or the precise localization of single molecules. Overall, these new approaches have created unprecedented new possibilities to investigate the structure and function of cells.

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Methods Mentioned

BETA
light microscopy
fluorescence imaging
electron microscopy
fluorescence microscopy
super-resolution microscopy
confocal microscopy

Software Mentioned

iPALM
dSTORM
Leica
GSDIM
SIM
isoSTED
STORM
PALMIRA

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