A Guinea Pig Model of Airway Smooth Muscle Hyperreactivity Induced by Chronic Allergic Lung Inflammation: Contribution of Epithelium and Oxidative Stress

Frontiers in Pharmacology
Luiz Henrique César VasconcelosBagnólia Araújo da Silva

Abstract

Asthma is a heterogeneous disease of the airways characterized by chronic inflammation associated with bronchial and smooth muscle hyperresponsiveness. Currently, different murine models for the study of asthma show poor bronchial hyperresponsiveness due to a scarcity of smooth muscle and large airways, resulting in a failure to reproduce smooth muscle hyperreactivity. Thus, we aimed to standardize a guinea pig model of chronic allergic lung inflammation mimicking airway smooth muscle hyperreactivity observed in asthmatics (Asth). Animals were randomly divided into a control group (Ctrl), which received saline (0.9% NaCl), and the Asth group, subjected to in vivo sensitization with ovalbumin (OVA) nebulization. Morphological analysis was performed by hematoxylin-eosin staining. Bronchial hyperresponsiveness was evaluated by nebulization time in the fifth, sixth, and seventh inhalations (NT5-7) and tracheal isometric contractions were assessed by force transducer. Total antioxidant capacity was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and protein expression by Western blot. Histologically, the Asth group developed peribronchial cellular infiltrate, epithelial hyperplasia and smooth muscle thickening. After the...Continue Reading

References

Apr 1, 1978·Agents and Actions·N Chand, P Eyre
Mar 1, 1990·The American Review of Respiratory Disease·T R Bai
Jan 1, 1986·Respiration; International Review of Thoracic Diseases·P J Barnes
May 1, 1988·The American Review of Respiratory Disease·A R Leff
Apr 1, 1985·British Journal of Pharmacology·F AhmedR C Small
Sep 1, 1995·American Journal of Respiratory and Critical Care Medicine·P J Barnes
Feb 1, 1993·The American Review of Respiratory Disease·N CarrollA James
May 1, 1997·American Journal of Respiratory and Critical Care Medicine·I F TibérioM A Martins
Jan 10, 2002·Pulmonary Pharmacology & Therapeutics·P A Henricks, F P Nijkamp
Apr 13, 2002·Japanese Journal of Pharmacology·Stefan MátyásViktor Bauer
Jun 18, 2003·Current Opinion in Pharmacology·Brendan J Canning
Jun 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Matthias SchmidtSabrina Mattoli
Mar 10, 2004·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·S J McMillan, C M Lloyd
Jul 20, 2004·Journal of Applied Physiology·Christopher J DanekAlan R Leff
Aug 17, 2004·The Journal of Allergy and Clinical Immunology·Stuart J HirstAlastair G Stewart
Apr 20, 2005·Internal Medicine Journal·K MorwoodF Kette
Jun 2, 2006·The European Respiratory Journal·A R RessmeyerC Martin
Aug 8, 2006·The Journal of Biological Chemistry·Wanglei DuJerry P Eu
Apr 28, 2007·American Journal of Respiratory and Critical Care Medicine·Lance CohenUNKNOWN NHLBI Severe Asthma Research Program (SARP)
Jun 22, 2007·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·G R Zosky, P D Sly
Mar 25, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Patrícia AngeliIolanda F L C Tibério
Apr 18, 2008·European Journal of Pharmacology·Reinoud GosensHerman Meurs
Jun 10, 2008·Mini Reviews in Medicinal Chemistry·Paolo Montuschi
Jul 1, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Nigel J FairbankGeoffrey N Maksym
Aug 2, 2008·The European Respiratory Journal·H MeursJ Zaagsma
Jan 13, 2009·Pulmonary Pharmacology & Therapeutics·H W Mitchell
Apr 24, 2009·Proceedings of the American Thoracic Society·Céline BergeronQutayba Hamid
Dec 17, 2009·Proceedings of the American Thoracic Society·Donna E Davies
Sep 21, 2011·Drug Discovery Today·Paolo Montuschi, Peter J Barnes
Nov 24, 2011·American Journal of Respiratory and Critical Care Medicine·Amanda SutcliffeChris E Brightling
Jul 10, 2012·Experimental Parasitology·Aleksandro S Da SilvaSilvia G Monteiro
Sep 13, 2012·Pulmonary Pharmacology & Therapeutics·David WrightAdrian R West
Sep 25, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Samantha Souza PossaIolanda de Fátima Lopes Calvo Tibério

❮ Previous
Next ❯

Citations

Feb 25, 2020·Oxidative Medicine and Cellular Longevity·Luiz Henrique C VasconcelosBagnólia A da Silva
Nov 30, 2019·Canadian Respiratory Journal : Journal of the Canadian Thoracic Society·Yiping LinYunguang Wang
May 4, 2021·Biological Trace Element Research·Farah AfrinLuqman A Khan

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis

Software Mentioned

ImageJ
Pro Plus
Image
Motic Plus
AQCAD
GraphPad
GraphPad Prism

Related Concepts

Related Feeds

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.