A gustatory receptor tuned to D-fructose in antennal sensilla chaetica of Helicoverpa armigera

Insect Biochemistry and Molecular Biology
Xiao-Jing JiangChen-Zhu Wang

Abstract

Insect gustatory systems play important roles in food selection and feeding behaviors. In spite of the enormous progress in understanding gustation in Drosophila, for other insects one of the key elements in gustatory signaling, the gustatory receptor (GR), is still elusive. In this study, we report that fructose elicits behavioral and physiological responses in Helicoverpa armigera (Harm) to fructose and identify the gustatory receptor for this sugar. Using the proboscis extension reflex (PER) assays we found that females respond to fructose following stimulation of the distal part of the antenna, where we have identified contact chemosensilla tuned to fructose in tip recording experiments. We isolated three full-length cDNAs encoding candidate HarmGRs based on comparison with orthologous GR sequences in Heliothis virescens and functionally characterized the responses of HarmGR4 to 15 chemicals when this receptor was expressed in Xenopus oocytes with two-electrode voltage-clamp recording. Among the tastants tested, the oocytes dose-dependently responded only to D-fructose (EC50 = 0.045 M). By combining behavioral, electrophysiological and molecular approaches, these results provide basic knowledge for further research on the m...Continue Reading

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Citations

Apr 10, 2016·Insect Biochemistry and Molecular Biology·Chao NingChen-Zhu Wang
May 2, 2016·Insect Biochemistry and Molecular Biology·Shingo KikutaRyoichi Sato
May 27, 2016·Insect Molecular Biology·A R AgnihotriR S Joshi
May 10, 2017·Natural Product Reports·Stefan PentzoldWilhelm Boland
Oct 13, 2017·PloS One·Tomoyuki TakadaShingo Kikuta
Aug 17, 2019·Microscopy Research and Technique·Fu LiuZhen Zhang
Dec 2, 2017·Archives of Insect Biochemistry and Physiology·Tomoyuki TakadaMaki N Inoue
Feb 27, 2021·Bulletin of Entomological Research·Lu-Lu LiYa-Nan Zhang
Jul 8, 2019·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Xiaoyi DouRussell Jurenka

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