A Gut Symbiotic Bacterium Serratia marcescens Renders Mosquito Resistance to Plasmodium Infection Through Activation of Mosquito Immune Responses

Frontiers in Microbiology
Liang BaiSibao Wang

Abstract

The malaria development in the mosquito midgut is a complex process that results in considerable parasite losses. The mosquito gut microbiota influences the outcome of pathogen infection in mosquitoes, but the underlying mechanisms through which gut symbiotic bacteria affect vector competence remain elusive. Here, we identified two Serratia strains (Y1 and J1) isolated from field-caught female Anopheles sinensis from China and assessed their effect on Plasmodium development in An. stephensi. Colonization of An. stephensi midgut by Serratia Y1 significantly renders the mosquito resistant to Plasmodium berghei infection, while Serratia J1 has no impact on parasite development. Parasite inhibition by Serratia Y1 is induced by the activation of the mosquito immune system. Genome-wide transcriptomic analysis by RNA-seq shows a similar pattern of midgut gene expression in response to Serratia Y1 and J1 in sugar-fed mosquitoes. However, 24 h after blood ingestion, Serratia Y1 modulates more midgut genes than Serratia J1 including the c-type lectins (CTLs), CLIP serine proteases and other immune effectors. Furthermore, silencing of several Serratia Y1-induced anti-Plasmodium factors like the thioester-containing protein 1 (TEP1), fibri...Continue Reading

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Citations

Jun 9, 2020·Frontiers in Microbiology·Krajana TainchumSylvie Manguin
Oct 24, 2020·Tropical Medicine and Infectious Disease·Elerson Matos RochaJayme A Souza-Neto
Apr 16, 2021·Journal of the American Mosquito Control Association·Yuebiao FengJingwen Wang
May 13, 2021·PLoS Biology·Daniel F Q SmithArturo Casadevall
May 24, 2021·Malaria Journal·Bianca E SilvaYael L Dahan-Moss
Jul 30, 2021·Frontiers in Microbiology·Blaire StevenDoug E Brackney
May 27, 2021·Molecular Ecology Resources·Hai-Jian HuangChuan-Xi Zhang

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Methods Mentioned

BETA
PCR
electrophoresis
CLIP
RNA-seq

Software Mentioned

HISAT
GraphPad
fq
GraphPad Prism
Cufflinks
Cuffdiff
filter

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